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ORFLO Technologies总部位于美国爱达荷州,拥有多项国际**技术,如微流控、生物信号向电子信号转化、数据采集和分析、配套分析软件设计等,**于现代细胞分析技术**和开发,在国际细胞分析技术竞争中处于**的地位。
ORFLOTechnologies 2010年开发****个掌上细胞计数仪,产品一经推出,很快受到业内广泛认可。目前推出*新代迷你细胞计数仪Moxi Z和掌上流式细胞仪MOXI FLOW。
世联博研(北京)科技有限公司正式做为Orflo中国代理商,主要产品包括Moxi Flow微型流式细胞仪及MoxiZ细胞计数产品系列等。
配套耗材:
一、Moxi Z 微型自动细胞计数仪配套芯片(Cell Counting
Cassettes)
芯片覆盖直径范围(2-34微米)内的所有细胞样品,无需根据细胞大小进行更换,
每个芯片可做2个测试。当样品1完成后,只需取出,将芯片的另一端插入Moxi Z,加入样品2。
在一次性盒式芯片中具有更高的性能和简单性。
Moxi Z独特的**保护薄膜细胞计数盒可在8秒钟内提供高精度,可重复的细胞计数和细胞大小分析(S型盒带15秒)。受全球**计数系统使用的金标准Coulter原理的启发,Moxi盒每个测试通过一个细胞感测区域流动数千个细胞,以准确捕获样品中每个单个细胞的基于阻抗的体积测量。基于图像的细胞计数方法,单个细胞的基于阻抗的细胞计数被证明是超过95%的准确度,而75%的准确度。
没有系统污染和集成预过滤器防止堵塞。
Moxi盒式芯片设计有独特的“细胞筛”,以*大限度地减少细胞结块和堵塞。另外,Moxi盒含有100%的样品在盒体内,消除了系统污染和**的可能性。
1、Moxi Z S型细胞计数芯片(Moxi Z Type S Cassettes)
The
Type S cassette is ideal for accurately measuring most yeast and other
particles as small as 3 µm and up to 20 µm in average diameter including some
algae and protozoa. Since the technology is based on a volumetric measurement,
non-spherical particles (14 - 4,200 fL) can also be measured accurately.
S型细胞计数芯片非常适用于**测量大多数酵母和其他颗粒,平均粒径小至3μm,直径达20μm,包括一些藻类和原生动物。 由于该技术基于体积测量,因此也可以准确测量非球形颗粒(14-4,200fL)。
Cell Counting Cassettes
Higher Performance & Simplicity in One Disposable Cassette.
Moxi Z's unique, patent-protected thin-film cell count cassettes provide highly accurate, repeatable cell counts and cell size analysis in under 8 seconds (15 seconds for Type S cassette). Inspired by the gold-standard Coulter Principle used in high-end counting systems worldwide, the Moxi cassette flows thousands of cells per test through a cell sensing zone to accurately capture the impedance-based volumetric measurement of each individual cell in the sample. Impedance-based cell counting of individual cells is proven to be over 95% accurate versus a 75% accuracy with image-based cell counting methods.
No system contamination and integrated pre-filter for clogging prevention.
Moxi cassettes are designed with a unique "cell sieve" to minimize cell clumping and clogging. In addition, Moxi cassettes contain 100% of your sample within the cassette body, eliminating the possibility for system contamination and sterilization. Cassettes are simply and easily discarded when testing is finished.
Moxi Z Type S Cassettes
The Type S cassette is ideal for accurately measuring most yeast and other particles as small as 3 µm and up to 20 µm in average diameter including some algae and protozoa. Since the technology is based on a volumetric measurement, non-spherical particles (14 - 4,200 fL) can also be measured accurately.
Moxi Z Type S Pack (25 Cassettes)
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Moxi Z Type S Box (250 Cassettes)
Moxi Z Type M Cassettes
The Type M cassette is ideal for accurately measuring mammalian cells or other particles 4-25 microns in average diameter. Since the technology is based on a volumetric measurement, non-spherical particles (34-8,180 fL) can also be measured accurately.
Moxi Z Type M Pack (25 Cassettes)
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Moxi Z Type M Box (250 Cassettes)
MOXI Z System Check Beads (5 mL)
Product Code: MXA005
MOXI Z System Check Beads (5 mL)
sh List
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Related Products
Accessories
Cassettes
Instruments
id
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MXA005
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Application Notes
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Can be used to check proper system function.
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Bead Diameter
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10 micron
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Compatible Instruments
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Moxi Z
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Concentration
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1.1 e+5 beads/mL
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Formulation
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Orflo Diluent
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Intended Use Statement
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For Research Use Only. Product is not for use in diagnostic procedures
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Material of Construction
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Polystyrene
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Overall Dimensions
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22.1 mm^3
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Sterility
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Non-sterile
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Weight
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5 mg
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Moxi Z Diluent
Product Code: MXA006
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Related Products
Reagents
Accessories
Cassettes
Instruments
id
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MXA006
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Application Notes
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Moxi Z Diluent is special formulated for use with the Moxi Z system for optimal sizing of particles and for operation with the instrument's Small Particle Mode (SPM).
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Applications
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Cell Dilution
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Applications Abbreviation
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Cdil
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Cassette Types
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Type M|Type S
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Compatible Instruments
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Moxi Z
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Formulation
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Proprietary
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Intended Use Statement
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For Research Use Only. Product is not for use in diagnostic procedures
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Kit Components
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One 100 ml bottle of diluent
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Number of Tests
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n/a
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Overall Dimensions
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52 L x 52 W x 106 H (mm)
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Protocol
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Pellet and resuspend cells in Moxi Z diluent or dilute sample with >90% Moxi Z diluent. 2. Pipette 10 uL of sample into tube 3. Pipette 90 uL of Orflo Diluent into tube 4. Gently mix resulting solution to ensure cells are evenly mixed 5. Pipette 75 uL of resulting solution into Moxi Z Cassette, or 50 uL into Moxi Flow cassette 6. Touch screen to run test
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Sample Type
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Mammalian Cells|Large Yeast|Large Algae|Protozoa
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Sterility
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Non-Sterile
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Storage Stability AND Handling
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Store at 2-8C. Use sterile technique when handling. Dispose of waste product in compliance with federal, state and local regulations. Do not inhale or ingest. Avoid contact with eyes and skin, flush affected area with water for at least 15 minutes
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Vendor
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ORFLO
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Weight
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152 g
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Moxi Z Diluent, sample
Product Code: MXA009
Moxi Z Diluent, 5 mL
See Product Specifications
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Related Products
Reagents
Accessories
Cassettes
Instruments
id
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MXA009
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Application Notes
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Moxi Z Diluent is special formulated for use with the Moxi Z system for optimal sizing of particles and for operation with the instrument's Small Particle Mode (SPM).
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Applications
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Cell Dilution
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Applications Abbreviation
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Cdil
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Cassette Types
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Type M|Type S
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Compatible Instruments
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Moxi Z
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Formulation
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Proprietary
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Intended Use Statement
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For Research Use Only. Product is not for use in diagnostic procedures
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Kit Components
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One 5 ml bottle of diluent
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Number of Tests
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75
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Overall Dimensions
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25 W x 45 H (mm)
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Protocol
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Pellet and resuspend cells in Moxi Z diluent or dilute sample with >90% Moxi Z diluent. 2. Pipette 10 uL of sample into tube 3. Pipette 90 uL of Orflo Diluent into tube 4. Gently mix resulting solution to ensure cells are evenly mixed 5. Pipette 75 uL of resulting solution into Moxi Z Cassette, or 50 uL into Moxi Flow cassette 6. Touch screen to run test
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Sample Type
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Mammalian Cells|Large Yeast|Large Algae|Protozoa
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Sterility
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Non-Sterile
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Storage Stability AND Handling
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Store at 2-8C. Use sterile technique when handling. Dispose of waste product in compliance with federal, state and local regulations. Do not inhale or ingest. Avoid contact with eyes and skin, flush affected area with water for at least 15 minutes
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Vendor
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ORFLO
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Weight
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11 g
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Orflo Accutaseª
Product Code: MXA020
Orflo Accutase cell detachment solution, 1X, 100 mL
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Related Products
Reagents
Assays
Antibodies
Cassettes
Instruments
id
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MXA020
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Application Notes
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Recommended for the detachment of adherent cells and dissociation of mammalilian cell lines and primary cells
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Applications
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Cell Detachment
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Applications Abbreviation
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Cdet
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Cassette Types
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Type M|Type S|Type MF-M|Type MF-S
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Cell Types Tested
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fibroblasts|keratinocytes|vascular endothelial cells|hepatocytes|vascular smooth muscle cells|hepatocyte progenitors|primary chick embryo neuronal cells|bone marrow stem cells|adherent CHO and HNK cells|macrophages|293 cells|L929 cells|immortalized mouse testicular germ cells|MIC5|3T3|Vero|COS|HeLa|NT2|MG63|M24 and A375 metastatic melanoma|gliomas U251|D54|HT1080 fibrosarcoma cells|HT29 colon cancer cells|Sf9 insect cells|human embryonic stem cells|human mesenchymal stem cells|and human neural stem cells.
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Compatible Instruments
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MoxI Z|Moxi Flow|zEPI Flow
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Formulation
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Accutase® enzymes in Dulbecco's PBS containing 0.5 mM EDTA and phenol red. 1X concentrate, no preservatives.
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Intended Use Statement
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For Research Use Only. Product is not for use in diagnostic procedures
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Kit Components
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One 100ml bottle of cell dissassociation reagent
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Number of Tests
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n/a
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Overall Dimensions
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52 L x 52 W x 106 H (mm)
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Protocol
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1. Place 100ml Accutase bottle in room temp water bath or thaw overnight in fridge 2. For Adherent Cells: a. Remove cell media b. Add 1.5ml Accutase per 25cm2 of flask area c. incubate at RT or 37C until cells detach (typically 5-15min) d. Pipette triturate to break apart clusters 3. For suspension cell aggregates: a. Pellet cells at 300xg, 5min b. Resuspend pellet in 1-2ml Accutase c. Incubate 15min d. Pipette triturate to break apart clusters 4. Count cells on the Moxi Z, Moxi Flow, or Zepi Flow (be sure to dilute samples into standard operating range of specific cassette, if they are above the stated concentration limits)
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Sample Type
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Primary Mammalian cells|immortalized adherent cells|non-ES cell types
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Sterility
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Sterile
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Storage Stability AND Handling
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Stable at -20C. After thawing can be stored for up to 2 months at 2-8C.
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Tradename
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ACCUTASE™ is a registered trademark of Innovative Cell Technologies
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Vendor
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ORFLO
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Weight
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159 g
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Orflo Accumaxª
Product Code: MXA021
Orflo Accumax, cell dissassociation reagent, 10X, 100 mL, sterile
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Related Products
Reagents
Assays
Antibodies
Cassettes
Instruments
id
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MXA021
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Application Notes
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Recommended for use with the Moxi Z and Moxi Flow platforms when working with clumpy aggregated cells. It is ideal for generating single cell suspensions from clumped cell cultures, detachment of cells from primary tissue and detachment of cells from plastic tissue culturware. Accumax is specifically designed for our combination fluorescence amp impedance based flow cytometers.
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Applications
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Cell Detachment
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Applications Abbreviation
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Cdet
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Cassette Types
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Type M|Type S|Type MF-M|Type MF-S
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Compatible Instruments
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MoxI Z|Moxi Flow|zEPI Flow
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Formulation
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10X proprietary solution of proteolytic, collagenolytic and DNase enzymes and does not contain mammalian or bacterial derived products.
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Intended Use Statement
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For Research Use Only. Product is not for use in diagnostic procedures
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Kit Components
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One 100ml bottle of cell dissassociation reagent
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Number of Tests
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n/a
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Overall Dimensions
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52 L x 52 W x 106 H (mm)
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Protocol
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1. Place 100ml Accumax bottle in room temp water bath or thaw overnight in fridge 2. For Adherent Cells: a. Remove cell media b. Add 1.5ml Accumax per 25cm2 of flask area c. incubate at RT or 37C until cells detach (typically 5-15min) d. Pipette triturate to break apart clusters 3. For suspension cell aggregates: a. Pellet cells at 300xg, 5min b. Resuspend pellet in 1-2ml Accumax c. Incubate 15min d. Pipette triturate to break apart clusters 4. Count cells on the Moxi Z, Moxi Flow, or Zepi Flow (be sure to dilute samples into standard operating range of specific cassette, if they are above the stated concentration limits)
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Sample Type
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Primary Mammalian cells|immortalized adherent cells|Human ES cells|Rodent ES
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Sterility
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Sterile
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Storage Stability AND Handling
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Stable at -20C. After thawing can be stored for up to 2 months at 2-8C.
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Tradename
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ACCUMAX™ is a registered trademark of Innovative Cell Technologies
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Vendor
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ORFLO
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Weight
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159 g
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