可重复利用的MB系列趋化小室产品可以用于趋化实验,肿瘤细胞的侵袭实验及细胞与细胞的相互作用的研究。
货号
面积(mm2)
上室体积(ul)
下室体积(ul)
滤膜规格
AP48
8mm2
50 ul
25ul
25 x 80mm 聚碳酸脂膜 25 x 80mm 硝酸纤维素膜
In the Neuro Probe AP48 chemotaxis chamber, chemoattractants added to the lower wells act on cells in a suspension added to the upper wells. A filter separates the upper and lower wells. After incubation the migrated adherent cells on the underside of the filter are stained and counted.
The AP48 consists of precision-machined acrylic top and bottom plates, assembly hardware, and a silicone gasket. The bottom plate has 48 wells, each with 25µL volume and a diameter of 3.2mm. Corresponding holes in the top plate form the upper wells when the chamber is assembled. A single 25 x 80mm piece of filter membrane is placed between the top and bottom plates, and a gasket is positioned over the filter to create a seal. Narrow raised rims around mating surfaces of top and bottom wells enhance the seal.
Before the migrated cells can be stained and counted, the nonmigrated cells must be wiped off the top side of the filter. An accessory pack (stock # P48AP)includes instruments for this purpose.
The AP48 is designed for use with polycarbonate and cellulose nitrate filters. Please note that we also sell the AC48, a 48-well chamber specially designed for use with cellulose nitrate filters
Accompanying each chamber is an AP48 protocol for its use and care.