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主营产品: Flexcell细胞力学和regenhu细胞3D生物打印机销售技术服务: 美国Flexcell品牌FX-5000T细胞牵张应力加载培养系统,FX-5K细胞显微牵张应力加载培养系统,Tissue Train三维细胞组织培养与测试系统,FX-5000C三维细胞组织压应力加载培养系统,STR-4000细胞流体剪切应力加载培养系统,德国cellastix品牌Optical Stretcher高通量单细胞牵引应变与分析系统 Regenhu品牌3D discovery细胞友好型3D生物打印机,piuma细胞纳米压痕测试分析、aresis多点力学测试光镊,MagneTherm细胞肿瘤电磁热疗测试分析系统
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neuroprobe现货ChemoTx system细胞迁移实验96孔板,ChemoTx® Disposable Chemotaxis System

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  • 产品名称:neuroprobe现货ChemoTx system细胞迁移实验96孔板,ChemoTx® Disposable Chemotaxis System
  • 产品型号:101-216
  • 产品展商:neuroprobe
  • 产品文档:无相关文档
简单介绍

ChemoTx system 细胞迁移实验96孔板用于细胞迁移实验的一次性产品,采用96孔板的标准形式,可以与目前的荧光酶标仪,和自动化分液器兼容使用。该系统包括趋化实验所用的所有组成:微孔板(1);膜滤器(1);盖子(1)。微孔板的小孔容量范围30ul-300ul。膜滤器的膜固定在板的外围边框上作为支撑,便于操作,膜滤器的每个测试位点有防水罩保护,限制了悬浮细胞移动到测试位点,这种设计不但对上、下两层小室中的细胞起了很好的隔离效果,并且减少了小室内水分的蒸发,和气泡的产生。

产品描述
 

ChemoTx system 细胞迁移实验96孔板

用于细胞迁移实验的一次性产品,采用96孔板的标准形式,可以与目前的荧光酶标仪,和自动化分液器兼容使用。该系统包括趋化实验所用的所有组成:微孔板(1);膜滤器(1);盖子(1)。微孔板的小孔容量范围30ul-300ul。膜滤器的膜固定在板的外围边框上作为支撑,便于操作,膜滤器的每个测试位点有防水罩保护,限制了悬浮细胞移动到测试位点,这种设计不但对上、下两层小室中的细胞起了很好的隔离效果,并且减少了小室内水分的蒸发,和气泡的产生。

 ChemoTx system产品---现货促销

 产品系列

101系列

106系列

111系列

116系列

微孔板的容量

30ul

30ul

300ul

300ul

测试位点滤膜暴露面积

8mm2

25mm2

8mm2

25mm2

适合的实验

适合大多数的荧光实验标准

荧光信号强度弱时,可以选择暴露面积大的滤器,孵育时间要比101系列延长3-4h

培养基量大时,选择孔容量大的板。

收集强的荧光信号。

另外,滤膜的孔径也是可以选择的有:2um3um5um8um10um12um。可参考滤膜产品信息介绍。

 

 

英文介绍

 

ChemoTx® Disposable Chemotaxis System

The ChemoTx® System is a disposable 96-well cell migration system. Based on the standard 96-well microplate format, it is compatible with most 96-well fluorescent and densitometric plate readers, as well as most liquid-dispensing robots.

The system consists of all the chamber components necessary to perform a basic chemotaxis assay: a microplate, filter, and lid. Plates are molded from tissue culture grade transparent polystyrene, with 30µL or 300µL well capacity.

 
  • Description
  • Additional Information

Product Description

The ChemoTx® System is a disposable 96-well cell migration system. Based on the standard 96-well microplate format, it is compatible with most 96-well fluorescent and densitometric plate readers, as well as most liquid-dispensing robots.

The system consists of all the chamber components necessary to perform a basic chemotaxis assay: a microplate, filter, and lid. Plates are molded from tissue culture grade transparent polystyrene, with 30µL or 300µL well capacity.

The filters for this system are available in a wide range of pore sizes. They are bonded to frames for ease of handling and selectively coated with a hydrophobic mask around each of the test sites. The hydrophobic mask restricts the cell suspension to these sites, eliminating the need for upper wells. Test sites are available in two exposed filter areas, 8mm2 or 25mm2. The hydrophobic mask allows pipetting of cell suspension directly onto the sites on the top side of the filter, where it sits in hemispherical drops, eliminating the need for upper wells. Additional hydrophobic coatings, combined with the proprietary design of the plates, provide the seal between the bottom of the filter and the rims of the plate, so there is no need for gaskets and clamping hardware. Bubble entrapment is minimized in both the top and bottom fluids by this design. The lid undefined in reducing evaporation and facilitates stacking.

There are a total of four possible combinations of plate well volume and exposed filter area. For most fluorescence assays, the 101 series, with 30µL plate well volume and 8mm2 filter area, works well. If the number of migrated cells does not create sufficient signal strength relative to the background, the 106 series, with 25mm2 area, will yield increased signal strength. If you will be incubating for longer than 3-4 hours, a unit with the larger, 300µL plate volume will allow for more media to sustain the cells. When quantifying the migrated cells with a densitometric reader, the 116 series will provide a larger exposed filter area for increased signal strength and deeper wells to simplify the staining process and enhance reading. If using the 300µL well volume is not desirable, e.g., when using rare or expensive reagents, an alternative is to use the 106 series with 30µL well volume for the migration and then use the 96-well funnel plate (FP1) to transfer the migrated cells into a deeper plate for staining and counting.

Polycarbonate membranes are available in a wide range of pore sizes. Information regarding pore density as it relates to pore size may be found on the Filters page. Find more information on Framed Filters here .

Plate & Filter Specifications 2µm 3µm 5µm 8µm 10µm 12µm
3.2mm dia. sites,
30µL 96-well plate
201-2* 101-3 101-5 101-8 101-10* 101-12*
5.7mm dia. sites,
30µL 96-well plate
206-2* 106-3 106-5 106-8 106-10* 106-12*
3.2mm dia. sites,
300µL 96-well plate
211-2 * 111-3 * 111-5 * 111-8 * 111-10 * 111-12 *
5.7mm dia. sites,
300µL 96-well plate
216-2* 116-3 116-5 116-8 116-10* 116-12*

2µm pore size has PVP treated membrane. Please contact us with any questions.

* Non-stock items – please call for availability.

 

Weight 0.25 lbs
Dimensions 7 x 6 x 2 in
Pore Size

2µm, 3µm, 5µm, 8µm, 10µm, 12µm

Well Capacity

300µL, 30µL

Cell Site Diameter

3.2mm, 5.7mm

Filter Membranes

Standard PCTE, PCTE with PVP

 

 

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