世联博研(北京)科技有限公司 主营:Flexcell细胞力学和regenhu细胞3D生物打印机销售技术服务: 美国Flexcell品牌FX-5000T细胞牵张应力加载培养系统,FX-5K细胞显微牵张应力加载培养系统,Tissue Train三维细胞组织培养与测试系统,FX-5000C三维细胞组织压应力加载培养系统,STR-4000细胞流体剪切应力加载培养系统,德国cellastix品牌Optical Stretcher高通量单细胞牵引应变与分析系统 Regenhu品牌3D discovery细胞友好型3D生物打印机,piuma细胞纳米压痕测试分析、aresis多点力学测试光镊,MagneTherm细胞肿瘤电磁热疗测试分析系统
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主营产品: Flexcell细胞力学和regenhu细胞3D生物打印机销售技术服务: 美国Flexcell品牌FX-5000T细胞牵张应力加载培养系统,FX-5K细胞显微牵张应力加载培养系统,Tissue Train三维细胞组织培养与测试系统,FX-5000C三维细胞组织压应力加载培养系统,STR-4000细胞流体剪切应力加载培养系统,德国cellastix品牌Optical Stretcher高通量单细胞牵引应变与分析系统 Regenhu品牌3D discovery细胞友好型3D生物打印机,piuma细胞纳米压痕测试分析、aresis多点力学测试光镊,MagneTherm细胞肿瘤电磁热疗测试分析系统
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CS-07-001a-xx,3D InSight Human Liver Maintenance Medium-AF(advanced formulation

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  • 产品名称:CS-07-001a-xx,3D InSight Human Liver Maintenance Medium-AF(advanced formulation
  • 产品型号:CS-07-001a-xx
  • 产品展商:瑞士Insphero
  • 产品文档:无相关文档
简单介绍

瑞士insphero公司生产的3D细胞悬滴培养板无需使用支架介质(scaffold-free),通过悬滴培养法(hanging-drop) ,就能将细胞悬液(一种细胞或多种细胞)形成3D微组织,3D微组织呈多细胞球状体,无论在形态学上,还是在功能上均与自然组织类似。

产品描述

瑞士Insphero品牌3D细胞悬滴培养板

3D(三维)细胞培养使细胞聚集生长,形成组织样结构,更好的模拟体内生理状况。瑞士insphero公司的3D细胞培养技术无需使用支架介质,通过悬滴

法给细胞提供一个更加接近体内生存条件的微环境,使细胞形成3D微组织,从而更好地模拟体内细胞生长情况,更直观的反映细胞生物学和功能,更准

确的构建靶组织模型。

 

两层平板=3D平台

上层平板称为:GravityPlusTM用于细胞培养,使其形成微组织(3D状态)

下层平板称为:GravityTRAPTM用于3D细胞(微组织)后续实验及检测

如下图所示:

      GravityPlusTM板中,一种细胞或多种细胞培养,使其形成微组织(球状);

      当微组织形成后,将其转移到GravityTRAPTM板中,用于后续实验培养和检测。

产品优势

无需支架介质,细胞可立体生长达数周

**的尺寸控制,孔间形成的微组织差异甚小

可用于标准化学发光和荧光检测,包括Promega ATPLDHGSH和细胞凋亡等指标

可用于高内涵分析(high content test)

与高通量加样设备兼容 

应用范围

可用于几乎任何类型细胞的3D培养,包括细胞系、原代细胞、干细胞及多种细胞共培养
癌症研究及**分析
细胞生物学研究和组织工程
可用于高内涵分析(high content test)
与高通量加样设备兼容
吸收、分布、新陈代谢、排泄及毒性测试(ADME-TOX)
**代谢动力学(DMPK

 订购信息

货号

名称

规格

CS-00-020

GravityPLUS 3D培养板

1x96

CS-06-001

GravityPLUS 3D培养板

10x96

瑞士Insphero品牌3D细胞悬滴培养板英文介绍

 

 

GravityTRAP™ ULA Plate

An enhanced, ultra-low attachment spheroid plate optimized for scaffold-free 3D cell culture-based assays

  • Cost-effective formation of scaffold-free spheroids via self-assembly in 96-well ultra-low attachment (ULA)-treated plates
  • SureXchange™ tapered ledge and culture chamber for easy medium exchange and spheroid visualization during long-term growth and analysis
  • 3D-optimized protocols available for analysis in GravityTRAP™ ULA Plate

 

  • Spheroid formation in GravityTRAP™ ULA Plates

    The GravityTRAP™ Ultra-Low Attachment (ULA) Plate represents a simple, flexible, and automation-compatible platform for the generation, long-term cultivation, observation and testing of scaffold-free 3D microtissue spheroids in 96 well format. Each plate consists of a special non-adhesively coated 96-well sterile-packaged GravityTRAP™ (Tissue Re-aggregation and Assay Plate), and lid.

    IMPORTANT
    InSphero recommends GravityTRAP™ ULA Plates for the generation of spheroids using immortalized cell lines that are known to readily form microtissues, or as a starting point for investigating whether or not a cell type can form self-aggregating, scaffold-free spheroids. InSphero recommends our patented GravityPLUS® Hanging Drop System (ISP-06-001,ISP-06-010) if generating spheroids in more complex 3D cell culture scenarios, such as when using primary cells, cell lines that are resistant to self-aggregation, or when generating co-culture microtissues (e.g., tumor/stroma). In such cases, the GravityPLUS® Hanging Drop System provides the greatest opportunity for success.

    GravityTRAP™ Ultra Low Attachment Plates Workflow

    Figure 1: Spheroid formation in the GravityTRAP™ ULA Plate begins with initial seeding of cells in suspension, followed by a brief spin to concentrate cells. Following microtissue maturation,the SureXchange™ ledge of the tapered well facilitates medium exchange and compound dosing without disturbing or losing the microtissue.

    Hey Tumor Spheroids at Day 3 After Seeding in Ultra Low Attachment Plates

    Figure 2: HCT-116 and Hey tumor spheroids at day 3 of culture following seeding in the GravityTRAP™ ULA Plate at various cell densitites, demonstrating consistent, spheroid shaped microtissue formation.

    Average Spheroid Size in Ultra Low Attachment Plates

    Figure 3:  Average size (day 3) of HCT-116 and Hey tumor spheroids as assessed by volume using the Cell3iMager. Standard deviation of 6 replicates per cell density shown for each cell line.

    Spheroid ATP Viability Comparison in Ultra Low Attachment Plates

    Figure 4:  Average ATP content of HCT-116 and Hey tumor spheroids as assessed by Promega CellTiter-Glo® assay. Standard deviation of 6 replicates per cell density shown for each cell line.

 

瑞士Insphero品牌3D细胞培养基

3D细胞悬滴培养板专用培养基

量身裁定的用于维持3D状态的培养基

即用型、无需混合和预处理

保证3D微组织可以培养数周时间

经优化、可直接用于3D InSight™微组织

 订购信息

·        

货号

名称

规格

CS-07-101

3D InSight™ 细胞系维持培养基

250/500mL

CS-07-100

3D InSightHepG2微组织维持培养基

500mL

CS-07-001

3D InSight™ 人肝细胞维持培养基

250/500mL

CS-07-002

3D InSight™ 大鼠肝细胞维持培养基

250/500mL

CS-07-005

3D InSight™ 人胰岛维持培养基

250/500mL

CS-07-006

3D InSight™ 大鼠胰岛维持培养基

250/500mL

 

 

 瑞士Insphero品牌3D细胞培养基英文介绍

3D InSight™ Cell-Culture Media

Optimized media for 3D cell culture – ready to use!

  • Safeguard your 3D microtissues in culture for weeks
  • Optimized for assay-ready 3D InSight™ Microtissues
  • Specialized media for tumor spheroid aggregation and maintenance
  • Ready-to-use, no mixing and preparation required

 

  • Catalog # Description
    CS-07-001a-xx 3D InSight™ Human Liver Maintenance Medium - AF (advanced formulation)*
    CS-07-001b-xx 3D InSight™ Human Liver Maintenance Medium - INF (for inflammatory tox applications)*
    CS-07-002-xx 3D InSight™ Rat Liver Maintenance Medium*
    CS-07-005-xx 3D InSight™ Human Islets Maintenance Medium*
    CS-07-006-xx 3D InSight™ Rat Islets Maintenance Medium*
    CS-07-100-01 3D InSight™ HepG2 Microtissue Maintenance Medium*
    CS-07-101-xx 3D InSight™ Cell Line Maintenance Medium


    NB: „xx" is the volume code: -01 for 500 ml and -02 for 250 ml. Please also inquire about customer-specific formulations.

 

 

 

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