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主营产品: Flexcell细胞力学和regenhu细胞3D生物打印机销售技术服务: 美国Flexcell品牌FX-5000T细胞牵张应力加载培养系统,FX-5K细胞显微牵张应力加载培养系统,Tissue Train三维细胞组织培养与测试系统,FX-5000C三维细胞组织压应力加载培养系统,STR-4000细胞流体剪切应力加载培养系统,德国cellastix品牌Optical Stretcher高通量单细胞牵引应变与分析系统 Regenhu品牌3D discovery细胞友好型3D生物打印机,piuma细胞纳米压痕测试分析、aresis多点力学测试光镊,MagneTherm细胞肿瘤电磁热疗测试分析系统
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低氧高氧常氧培养系统,低氧高氧常氧培养系统

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  • 产品名称:低氧高氧常氧培养系统,低氧高氧常氧培养系统
  • 产品型号:MIC-101
  • 产品展商:Billups-rothenberg
  • 产品文档:无相关文档
简单介绍

便携式细胞/微生物氧环境控制培养系统 ——氧气浓度控制(高氧、常氧、低氧)培养系统

产品描述



应用于体外受精、艾滋病毒的分离、建立低氧、高氧和正常含氧量的环境条件,器官培养和大量细胞株的培养。每一个单元都是一个独立的培养室,可以建立所需的培养室拥有良好的气密性和快速的气体交换特性,快速经济的形成一个稳定组织培养环境(包括气体浓度和湿度)。

MIC-101培养室


• 三层支架,空闲充裕

主体材质是透明的聚碳酸酯,有非常高的透视性和耐久性
• 培养室可以堆叠摆放、内扣连接后振荡培养,作为储存室节省空间
• 内置多用途托盘,方便放置培养皿、试管、组织培养瓶、微量板等
• 方便简单的盖子和 Marman 夹设计,使实验操作便捷
• 所有组件都无毒,可酒精**
• 培养室内部夹角无缝隙连接便于清洗和去污
• 圆柱腔的几何形状提供了*好的气体分布
•O 型圈有着**的气密性密封

尺寸
外径:直径 31cm 高 11cm
内径:26.7cm
板间间隙:2.3cm
容量
84 个 35mm 培养皿
27 个 60mm 培养皿
12 个 100mm 培养皿
9 个 96 孔培养板
18 个 25cm2 组织培养瓶


流量计

流量计包括单流量计和双流量计 2 种,能帮用户确定知道何时获得 100%气体交换,流量可在 3~25L/min(或 0.1~10LPM)之间任意调节。

单流量计被用来控制预混合气体的流率;

双流量计除了控制气体流率还可以用来混合气体(双流量计被用作检测与氧气检测器组合使用)。

每个培养室的体积大约为 8L。
• 单流量计用于控制预混合气体的流速时为每分钟 1.0~25L (或 0.1~10LPM

双流量计通常用于需要对 O2 和 CO2 的浓度进行调整以确定气体混合后效果的实验。此系统有两个流量计,一个为 0.1~1.0LPM,另一个为 0.1~10LPM, 有两个不同的管口,允许气体从两个不同的气瓶输入。当流量计为 0.1-1.0LPM 流量计的进气口被关闭时,双流量计可以以相同的方式作为单个流量计可用于预混合气体。

使用本设备发表的高水平文章
1. Two Novel Missense Mutations of the HFE Gene (I105T and G93R) and Identification of the S65C Mutation in Alabama Hemochromatosis Probands* 1
[PDF] from lbtek.comJC Barton, R Sawada-Hirai, BE Rothenberg… - Blood Cells,
Molecules, …, 1999 - Elsevier
... Probands *1. James C. Barton a , f1 , Ritsuko Sawada-Hirai b , Barry E. Rothenberg
c and Ronald T. Acton c. a Southern Iron Disorders Center, Birmingham, Alabama. b
Billups-Rothenberg, Inc. San Diego, California. c Immunogenetics ...
Cited by 150 - Related articles - BL Direct - All 14 versions
2. Preconditioning of the tumor vasculature and tumor cells by intermittent hypoxia: implications for anticancer therapies
[HTML] from aacrjournals.orgP Martinive, F Defresne, C Bouzin, J Saliez, F Lair… - Cancer research, 2006 - AACR
... To reach and control hypoxia conditions, cells were placed in a modular incubator chamber (Billups Rothenberg, Inc., Del Mar, CA) and flushed for 10 minutes with a gas mixture of 5%CO2 -95% N 2 ; the final medium pO 2 value was consistently measured below the 0.5% to 1 ...
Cited by 61 - Related articles - BL Direct - All 9 versions
3. The pH-dependent membrane association of procathepsin L is mediated by a 9-residue sequence within the propeptide.
[PDF] from jbc.orgGF McIntyre, GD Godbold… - Journal of Biological Chemistry, 1994 -
ASBMB.. inhibitor E-64, dithiothreitol, and HEPES were from Boehringer Mannheim; ECL Western Blotting Detection Re- agents and donkey anti-rabbit serum
conjugated to horseradish peroxi- dasewere from Amersham Corp.; the HotBox System was from Billups- Rothenberg, Del Mar ...
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4. Intersection of interferon and hypoxia signal transduction pathways in nitric oxide-inducedtumor apoptosis
[PDF] from lbtek.comDS Tendler, C Bao, T Wang, EL Huang… - Cancer research, 2001 -
AACR.. NAME, or a combination of these factors. Cells were cultured under normoxia or
hypoxia (5% CO 2 and 95% N 2 ) in an Incubator Chamber (Billups-Rothenberg, Del Mar, CA), and were harvested after incubation for up to 24 h. ...
Cited by 50 - Related articles - BL Direct - All 7 versions
5. Beta2 knockout mice develop parenchymal iron overload: A putative role for class I genes of the major histocompatibility complex in iron metabolism
[PDF] from pnas.orgBE Rothenberg… - Proceedings of the National …, 1996 - National Acad Sciences ... To whom reprints should be sent at the present address: Billups- Rothenberg, Inc., POBox 977, Del Mar, CA 92014. 1529 Page 2. 1530 Medical Sciences: Rothenberg andVoland Proc. Natl. Acad. Sci. USA
93 (1996) A -200 -175 -150 125 ...
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6. Genetic and Clinical Description of Hemochromatosis Probands and Heterozygotes: EvidenceThat Multiple Genes Linked to the Major Histocompatibility
Complex …
[PDF] from scripps.eduJC Barton, WWH Shih, R Sawada-Hirai…- Blood Cells, Molecules, …,1997 - Elsevier... c and Barry E. Rothenberg b. a Southern Iron
Disorders Center and Brookwood Medical Center, Birmingham, Alabama. b Billups-Rothenberg, Inc. San Diego,California. c Immunogenetics Program, Departments ofMicrobiology ...
Cited by 138 - Related articles - BL Direct - All 10 versions
7. Isoflurane neuroprotection in hypoxic hippocampal slice cultures involves increases in intracellular Ca2+ and mitogen-activated protein kinases
[PDF] from lbtek.comJJ Gray, PE Bickler, CS Fahlman, X Zhan… - …, 2005 -
... Experiment Design. Cultures were exposed to hypoxia by placing them into a 2-l airtight Billups-Rothenberg Modular Incubator Chamber (Del Mar, CA)
through which 95% N2 –5% CO 2 gas, preheated to 37°C, was passed at 5–10 l/min. ...
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8. Limitations to oxygen diffusion and equilibration in in vitro cell exposure systems in hyperoxia and hypoxia
[HTML] from physiology.orgCB Allen, BK Schneider… - American Journal of …, 2001 - Am
Physiological Soc... One assembly with an intact top and one with a perforated top were placed in a polystyrene environmental chamber with an internal volume
of 5.72 liters (Billups-Rothenberg, Del Mar,CA), and the electrical connections for the electrodes were passed out through the gas ...
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9. In vivo genetic profiling and cellular localization of apelin reveals a hypoxia-sensitive,endothelial-centered pathway activated in ischemic heart failure
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N 2 , and sealed before placement in a tissue culture incubator. ...
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... The cells were then placed into a 37 °C humidified incubator (normoxia) or into a
modular incubator chamber (Billups Rothenberg, Del Mar, CA) and flushed for 20
min with a mixture of 1% O 2 , 5% CO 2 , and 94% N 2 (hypoxia). ...
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