BiospaceLab PhotonIMAGER小动物活体光学成像系统
小动物活体光学成像系统（photonimager）是市场上**的一款能够动态收集和定量检测生物体内和体外荧光与生物发光信号的成像系统。
一个体外内均可独特的模块化用于检测、定位、定量动态生物荧光或荧光信号仪器
产品特点：
* 3D/4D成像
*s事实显微观察
*多谱段的分析成像
* X-射线成像
* 实时生物发光和荧光成像系统
* 模块化的仪器用于实时检测、定位、定量分析体内或体外的生物发光或荧光信号
* 光谱范围宽，从蓝光到近红外光，激发光波长从450-1000nm
* 从个体到细胞水平
* 高灵敏度，加强型CCD相机，无需更长时间曝光和像素Binning，可检测微弱信号
* 23ms的高时间分辨率
* **的信号定位和定量
* 检测窗口可选整个动物或某一解剖部位
* 实时信号获取可分析每个样本的发射光峰值时间
* 模块化设计方便添加或升级模块：
* Macrolens 模块用于更高分辨率成像
* Macro2Micro 模块用于微米级高分辨率成像
* Multispectral 模块用于多光谱成像
* 4-View 模块可从4个角度观察图像
* X-Ray 模块用于解剖部位定位
* In Actio? 模块用于自由活动动物的动力学成像
* 操作方便
* 温控平台和麻醉系统方便操作动物
* 高通量纵向研究系统，*多可同时处理5只小鼠
应用：
生物体内分布
癌症研究
基因表达研究
传染病研究
神经学
药代动力学
干细胞研究
技术参数：
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PhotonIMAGER RT
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PhotonIMAGER Optima
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照相机
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传感器
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加强型CCD
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加强型CCD
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镜头
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24mm,f/1.4-22
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50mm,f/1.2-16
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运行温度
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-25℃
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-25℃
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性能
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检测波长范围
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370-900nm
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370-900nm
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时间分辨率
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23ms
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23ms
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视野范围
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*小3.4×2.8mm
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*小3.4×2.8mm
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*大25.5×18cm
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*大31.5×23.5cm
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光源
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150W卤素灯
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150W卤素灯
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荧光
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多种波长可选
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多种波长可选
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滤光片
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激发光滤光片
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450-750nm
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450-1000nm
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发射光滤光片
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6个
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10个
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混合像元分解
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有
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有
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多种标记功能
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有
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有
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动物操作
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气体麻醉
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有
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有
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加热平台
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25℃-45℃
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25℃-45℃
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成像室尺寸
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25×26×38cm(W×D×H)
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50×40×70cm(W×D×H)
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模块
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动态成像
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有
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有
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同时多角度观察
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有
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有
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3D重建软件
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无
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有
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2D X射线分析和定位
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无
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有
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In Actio分析和定位
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有
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有
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系统要求
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操作系统
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Windows XP/7
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Windows XP/7
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电源
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1KW 150或230V
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1KW 150或230V
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尺寸
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60×75×105cm(W×D×H)
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60×85×140cm(W×D×H)
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重量
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85kg
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140kg
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Biospace Lab 前身为Biospace measures 公司，由诺贝尔物理学奖得者乔治夏帕克教授根据其在高能物理和粒子检测方面的研究发现，于1989年组建成立。乔治夏帕克教授及其团队**于生物影像工具的研究开发。公司成立以来，积*与医学、药学和其它领域的科研机构合作，力求满足用户对生物医学影像仪器的实际需求。目前，Biospace Lab已经发展了多种用于小型动物成像仪器，并拥有自己的**技术。
Biospace Lsb**于高性能临床前实验影像仪器的研制，成功开发出用于小型动物体内、体外和活动动态成像的成像系统。目前，该成像系统已经在世界知名**公司和权威研究中心投入使用。
Biospace Lab的研发和生产总部位于法国巴黎，在欧洲、北美和亚洲均设有分公司。所有产品，包括其设计、生产、销售、安装和售后服务均符合ISO9001：2000认证规范。
Biospace Lab光学活体成像工作原理：
使用Intensified CCD成像，即在原来CCD的基础上装上增强光信号的信号增强管。该装置包括：光电阴*、微通道板（microchannel plate）和荧光屏。
光源通过物镜聚焦后投射到光电阴*，因为光电效应，投射到光电阴*的光子转变为电子，让该电子通过一个强电场，使电子通过MCP后变成携带高能量的电子云，这些电子云投射到荧光屏便形成肉眼可见的光斑。这样，ICCD就能轻易捕捉到单一光子成像。
如右图所示。
Biospace Lab光学活体成像优点：
● 使用ICCD成像，有效提高信噪比，消除噪音对结果的干扰，在Photon成像系统中，相机的噪音减少到7 x 10-5 e-/像素 or或35 e-/s/cm2
● 对实验动物进行实时观察，全界面清晰成像，无需理会光圈、焦距的使用，不必摸索拍摄曝光时间，结果肉眼随时可视，所得的数据更加真实可信。
● 拥有Biospace Lab**数据成像模型：Enhanced Counting Mode，使该系统动态检测范围灵敏度提高到5个数量级，达50 000 光子/s/mm2.
● 利用系统兼容软件M3Vision进行全自动数据分析
世联博研北京科技有限公司代理BiospaceLab的产品一共有五种：
The Photon Imager；
The Micro Imager；
The Beta Imager；
The Gamma Imager；
Beta MicroProbe。
详情请致电世联博研北京科技有限公司国内免费客服专线：400-650-8506
除了覆盖一般要求的冷光及荧光成像外，BiospaceLab还有更**的放射性同位素成像和微探针系统,这在目前是暂时没有竞争对手的。
特点：采用第3代ICCD系统可将*微弱的电场信号放大100万倍，而背景值比传统冷CCD低100~1000倍，这是技术优势所在。
系统只要加入模块，无需改变结构，就可以增强或改进成像系统的功能,可根据实验情况逐步升级，让能效比*大化.升级潜力大，同时亦延长了仪器的折旧报废周期，让您的投资物有所值.
APPLICATION NOTES
PhotonIMAGER systems
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Cerenkov Luminescence imaging on the PhotonIMAGER? system -
biodistribution of the beta emitting radiotracer 32P
Cerenkov imaging is shown to be a valid tool for complementing more commonly used BLI, FLI, SPECT and PET imaging modalities. This study successfully demonstrated how visualization of tumor onset/progression can be achieved by detecting Cerenkov luminescence from injected 32P radiolabel. The PhotonIMAGER? system is shown to be perfectly adapted to monitor Cerenkov Luminescence in vivo.
Download pdf
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The importance of Real-Time acquisition for identifying the signal plateau in bioluminescent in vivo imaging
The emission of light following the reaction between Luciferase and it’s substrate Luciferin is the result of an enzymic reaction. The signal dynamics following following Luciferin injection in vivo will therefore be strongly dependant upon many factors; including temperature, pH, and location of Luciferase-expressing cells in vivo. This application note demonstrates the advantages of Real-Time signal acquisition for in vivo optical imaging.
Dowload pdf
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Macrolens Module: detecting metastasis in mouse lung
It has been demonstrated in this study that it is possible to resolve metastasis in the lung ex vivo at higher magnification, even when they cannot be resolved in vivo during whole body imaging.
The Macrolens module also allows precise localization of closely spaced signals making it possible to reliably quantify each one.
Download pdf
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Real -Time monitoring of luminescent nanoprobe biodistribution
The biodistribution of luminescent nanoparticles can be controlled by altering their chemical surface and electrical charge. They can then be monitored in Real-Time using the PhotonIMAGER. This is of particular interest for pharmacological applications.
Download pdf
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In Actio Module: Real-Time bioluminescence imaging in freely moving small animals
This study demonstrates the performance of a device developed for whole body imaging of small moving animals at high time resolution. The system comprises a camera for photon counting of bioluminescence signal and a video monitoring function to track the animals movements. The In Actio Module allows Bioluminescence imaging where anesthetics are suspected to cause physiological interference, such as studies monitoring tumor growth.
Download pdf
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PhotonIMAGER?4-view module:
A device for simultaneous view of all the sides of animal body
The 4-View module is a device dedicated to the whole body imaging. Using thismodule reduces time needed to visualize allthe signals. This tool allows both bioluminescence and fluorescentimaging in real time. With this module problems which might appear due topositioning of the signal in the animal find their solution. The 4-View moduleis especially useful when signal appears on lateral sides and is not alwaysclearly visible with the standard module.
Download pdf
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Leishmania Infantum infection monitoring by bioluminescence
This study shows that Bioluminescence imaging allows to obtain as much information as standard in vitro techniques concerning the evaluation of the parasite charge. Moreover, the PhotonIMAGER is well indicated for longterm monitoring of parasites plague without sacrificing the animals.
Download pdf
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Marteau et al, 2013, Angiopoietin-2 is vasoprotective in the acute phase of cerebral ischemia
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Abstract
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Abstract
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Abstract
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Abstract
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Ping et al, 2013, FGFR-targeted gene delivery mediated by supramolecular assembly between b-cyclodextrin-crosslinked PEI and redox-sensitive PEG
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Hombach et al 2013, Adoptive Immunotherapy with Redirected T Cells ProducesCCR7- Cells That Are Trapped in the Periphery and Benefit from Combined CD28-OX40 Costimulation
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