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主营产品: Flexcell细胞力学和regenhu细胞3D生物打印机销售技术服务: 美国Flexcell品牌FX-5000T细胞牵张应力加载培养系统,FX-5K细胞显微牵张应力加载培养系统,Tissue Train三维细胞组织培养与测试系统,FX-5000C三维细胞组织压应力加载培养系统,STR-4000细胞流体剪切应力加载培养系统,德国cellastix品牌Optical Stretcher高通量单细胞牵引应变与分析系统 Regenhu品牌3D discovery细胞友好型3D生物打印机,piuma细胞纳米压痕测试分析、aresis多点力学测试光镊,MagneTherm细胞肿瘤电磁热疗测试分析系统
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现货 4582,IncuCyte™ ClearView 96-Well Chemotaxis Plate

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  • 产品名称:现货 4582,IncuCyte™ ClearView 96-Well Chemotaxis Plate
  • 产品型号:4582
  • 产品展商:essenbioscience
  • 产品文档:无相关文档
简单介绍

4582 IncuCyte™ ClearView 96-Well Chemotaxis Plates 1 plate 4599 IncuCyte™ ClearView 96-Well Chmotaxis Plates – Case of 50 Plates 50 plates 4600 IncuCyte™ ClearView Reservoir Plate – 10 Pack 10 plates 4601 IncuCyte™ ClearView Reservoir Plate – Case of 100 Plates 100 plates

产品描述

IncuCyte™ ClearView 96-Well Chemotaxis Plate

Combine the optical clarity of microfluidics devices with the throughput of transmembrane assays. Each well of a ClearView plate provides an optically clear surface for label-free imaging and analysis of chemotactic cell migration using the IncuCyte ZOOM® Live-Cell Imaging Instrument.


Key advantages

Visualize every cell

  • The specially designed ClearView membrane enables you to monitor every cell throughout your experiment in real time – gain deep phenotypic insight into cell movement and morphology

Monitor every cell in your experiment. The ClearView membrane enables label-free visualization and quantitation of chemotaxis using IncuCyte ZOOM®. Each membrane contains 96 precision engineered holes in a regular grid array. Three pores are highlighted in yellow.


Low cell usage ideal for rare, expensive and primary cell populations

  • Use 10- to 100-fold fewer cells than standard transmembrane (e.g. Boyden Chamber) approaches

Lower cell usage. Cell usage comparison for the ClearView Plate and Corning® Transwell® system. Jurkat T cell chemotaxis toward SDFα-1 was measured in both systems (2,000 to 20,000 cells/well); however, no signal was observed with the Transwell® plate. A minimum of 150,000 cells/well was required to observe chemotactic migration using the Corning® Transwell® system.


Biologically relevant surface-mediated migration

  • Cells must migrate across a biologically relevant surface towards the chemoattractant due to the low pore density of the specially designed ClearView membrane (pore density <2% that of Boyden chamber transwells)
  • The ClearView plate has been validated with a wide range of biomatrix coatings, cell types and chemoattractants

Cells must interact with substrate to reach a pore. Time-lapse images of a Jurkat T cell (outlined in white) migrating across a Matrigel® coating towards a pore leading to the chemoattractant SDF-1α (orange circle). White arrows indicate the direction of Jurkat movement in each frame. In the final image the Jurkat T cell has entered the pore and moved to the lower chamber.


Stable and sustained gradients

  • Chemotactic gradients are stable and maintained over 72 hours

Long-term stable gradients compared to traditional Boyden chamber transwells. (A) Gradient stability was monitored over 72 hours using an Alexa Fluor® 594 dextran (10 kDa). After 4 hours a rapid loss in gradient was measured in the Corning® Transwell® system. In contrast, >80% of the gradient was maintained in the IncuCyte™ ClearView plate at 72 hours. (B) FBS gradients pre-establised in a ClearView plate for 24, 48, and 72 hours induced equivalent rates of directional migration in HT1080 cells indicating long-term and stable gradients across the ClearView membrane. Rates were slightly faster than for cells migrating towards gradients that had not been pre-established. This is likely a result of increased cell health when serum starved cells are plated in established gradients.


Compatible with standard automation and liquid dispensing devices

  • 96-well format suitable for screening workflows
  • Compliant with SBS microtiter plate requirements

Comparison of the ClearView Chemotaxis Plate with existing Boyden chamber transwell and microfluidic approaches

ClearView Boyden Chambers Microfluidic Devices
Visually observe cell
movement and morphology
Label-free analysis
Pore density Low High N/A
Stable chemotactic gradient
Kinetic read-out
Low cell usage (< 5,000 per well)
Surface interactions– Integrin sensitivity
96-well throughput or higher
Workflow: set up and walk away
Automation friendly

Overview of the ClearView Chemotaxis Plate design

See what's in the box 

The IncuCyte™ ClearView Chemotaxis Plate combines the throughput of transmembrane assays with the optical clarity of microfluidics devices. Each well of a ClearView plate provides an optically clear surface for label-free imaging and analysis of chemotactic cell migration using the IncuCyte ZOOM® Live-Cell Imaging Instrument.

The ClearView membrane contains 96 laser etched pores with a diameter of 8µm. Cells are added to the upper chamber and chemoattractant to the lower reservoir plate. Chemotactic transmembrane migration is visually monitored within your incubator and automatically analyzed using the IncuCyte ZOOM® Live-Cell Imaging Instrument.

Plate composition

Each IncuCyte™ ClearView Cell Migration Plate is composed of :

  • One plate lid (A)
  • One 96-well ClearView membrane insert (B)
  • One 96-well reservoir plate (C)

Product specs & configuration

  • Tissue culture treated
  • PET membrane insert
  • Insert area is 0.048 cm2
  • 8 µm pores
  • <2% pore density of Boyden chamber transwells
  • 96-well

Ordering Information

Catalog No. Product name Qty
4582 IncuCyte™ ClearView 96-Well Chemotaxis Plates 1 plate
4599 IncuCyte™ ClearView 96-Well Chmotaxis Plates – Case of 50 Plates 50 plates
4600 IncuCyte™ ClearView Reservoir Plate – 10 Pack 10 plates
4601 IncuCyte™ ClearView Reservoir Plate – Case of 100 Plates 100 plates

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Resources

  • IncuCyte™ Chemotaxis FAQs
  • Data Processing Tech Note
  • IncuCyte™ Chemotaxis Protocols
  • IncuCyte™ Chemotaxis Cell Migration Brochure [PDF]

Learn more about related products

  • IncuCyte™ Chemotaxis Software
  • Scratch Wound Migration and Invasion
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