Cellaxess® Elektra Discovery Platform

1.  产品介绍：

Cellaxess Elektra Discovery Platform在细胞发育的任何时期原位操纵和监控黏附细胞的情况，该系统突破了在应用药理学和生命科学研究领域的瓶颈， 实现了以下3个方面的研究：

小分子监控：细胞培养液的刺激和电压控制

染色体监控：原代细胞和干细胞的siRNA和cDNA的转染

主要影响因子和靶细胞的确认：转运小分子、抗体和多肽到细胞内

2.  特点

• 瞬间高通量的转染方法

• *少量的DNA或RNA去转染*多的细胞

• 几乎为零对细胞的毒性、脱靶效应和**反应.

• 成本低，高效率原处转染细胞

• 适用任何底物和细胞浓度

• 维持细胞的形态和活力

• 细胞发育的任何时期

• HCA兼容96孔和384孔细胞培养板

• 实时记录瞬间细胞发生的各种变化

•  配置高分辨率、高速率的采集图片机

3.  应用

3.1 转染原代细胞和诱导的多功能性干细胞

Cellaxess Elektra可以在保持低细胞损耗以及高灵活度的条件下，原处转染神经元和其他黏附细胞。这方面的应用包括：

l  染色体监控

l  瞬间转染过量表达的靶细胞

通过细胞培养中基因调控制作**模型

3.2细胞内化合物转运和靶细胞监控

Cellaxess Elektra是一项强大的化合物监控技术，使细胞任何化合物内从小分子到多肽到抗体实现高通量转运。

3.3电场刺激对功能的影响

Cellaxess Elektra还可以对黏附细胞和组织在原处实现高通量电刺激，与此同时通过荧光度动态变化实时监控反应电场处理对其的作用。Cellaxess Elektra也是研究电场刺激对兴奋细胞作用的**的方法，目前已经成功应用在**神经系统和心血管系统研究中。主要试验包括：

l  细胞信号试验

l  分泌试验

l  电压控制试验

 The Cellaxess Elektra is a revolutionary automated platform for the in-situ electric field manipulation of primary adherent cells and stem cells. The platform is highly flexible, and can be used for cell stimulation, general intracellular delivery and transfection across a diverse range of applications in cell biology and biomedical research.

Combining the Cellaxess Elektra’s ability to electrically stimulate adherent cells with dynamic imaging, allows Cellectricon to perform high quality phenotypic assays for compound characterization and target validation in a high-throughput format.

Electrostimulation pulses are delivered to cells via capillary electrodes enabling the direct manipulation of cells in HCA compatible 96- or 384- well plates. The focused nature of the electromanipulation minimises electrochemical toxicity and joule heating, to guarantee retained cell morphology and excellent cell viability.

The Cellaxess Elektra system is equipped with an imaging-based microplate reader to simultaneously record transient fluorescence and luminescence signals, providing real-time monitoring and information-rich readouts of cell culture response.

Publications

All Cellaxess Dynaflow Company Related

Please click on the product buttons above right, to filter the publications to suit your requirements. Note: Cellaxess®HT was the previous name for Cellaxess® Elektra which has additional functionality for new applications.

Cellaxess

Mitotic Motors Coregulate Microtubule Patterns in Axons and Dendrites Author(s):

Shen Lin 1, Mei Liu 1,2, Olga I. Mozgova 1, Wenqian Yu 1, and Peter W. Baas 1,2

1) Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, Pennsylvania 19129, USA
2) Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, China

Published in: The Journal of Neuroscience, (2012) , 32(40): 14033-14049

High-Throughput Transfection of Differentiated Primary Neurons from Rat Forebrain Author(s):

Shane Marine 1, Jamie Freeman 2, Antonella Riccio 2, Marie-Louise Axenborg 3, Johan Pihl 3, Robin Ketteler 2, and Sara Aspengren 3*

1) Department of Automated Biotechnology, Merck & Co., Inc., North Wales, PA, USA
2) MRC LMCB, University College of London, London, UK
3) *Cellectricon AB, Mölndal, Sweden, Email: sara.aspengren@cellectricon.se

Published in: Journal of Biomolecular Screening | March 8, 2012

Ef?ciency of Cellular Delivery of Antisense Peptide Nucleic Acid by Electroporation Depends on Charge and Electroporation Geometry Author(s):

Mette Joergensen 1, Birgit Agerholm-Larsen 1,2, Peter E. Nielsen 3, and Julie Gehl 1.

1) Department of Oncology, Copenhagen University Hospital Herlev, Herlev, Denmark
2) Department of Neurology, Copenhagen University Hospital Glostrup, Glostrup, Denmark
3) Department of Cellular and Molecular Medicine, Health Science Faculty, University of Copenhagen, Copenhagen, Denmark

Published in: OLIGONUCLEOTIDES, Volume 21, Number 1, 2011

Cellaxess®HT cell-based assay and transfection lab Author(s):

Sara Aspengren*, Michael Tokarz & Johan Pihl

*Cellectricon AB, Flöjelbergsgatan 8C, SE-431 37 Mölndal, Sweden

Published in: NATURE METHODS | JUNE 2011

An NGF-responsive element targets myo-inositol monophosphatase-1 mRNA to sympathetic neuron axons Author(s):

Catia Andreassi, Carola Zimmermann, Richard Mitter, Salvatore Fusco1, Serena De Vita, Adolfo Saiardi, & Antonella Riccio*

*Department of Neuroscience, Physiology and Pharmacology, University College London, London, UK

Published in: Nature Neuroscience 13, 291 - 301 (2010)

Transfection of chicken cerebellar granule neurons used to study glucocorticoid receptor regulation by nuclear receptor 4A (NR4A Author(s):

Strøm BO, Aden P, Mathisen GH, Lømo J, Davanger S, Paulsen RE.

Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, Oslo, Norway.

Published in: J Neurosci Methods. 2010 Oct 30;193(1):39-46. Epub 2010 Aug 19

FUNCTIONAL DIFFERENCES BETWEEN D1 AND D5 REVEALED BY HIGH RESOLUTION IMAGING ON LIVE NEURONS Author(s):

M. KRUUSMÄGI, S. KUMAR, S. ZELENIN, H. BRISMAR, A. APERIA AND L. SCOTT*

*Department of Women’s and Children’s Health, Karolinska Institutet, Stockholm, Sweden

Published in: Neuroscience 2009, 164, 463–469

Cellaxess®HT: high-throughput transfection for genomewide RNAi Author(s):

Johan Pihl*, Marie-Louise Johansson, Daniel Granfeldt, Michal Tokarz, Mattias Karlsson & Jon Sinclair

*Cellectricon AB, Flöjelbergsgatan 8C, SE-431 37 Mölndal, Sweden

Published in: Nature Methods - 5, (2008)

Generation of Focused Electric Field Patterns at Dielectric Surfaces Author(s):

Jessica Olofsson(1), Mikael Levin(2), Anette Strömberg(2), Stephen G. Weber(3), Frida Ryttsén(2), and Owe Orwar(1)
1) Department of Chemistry and Bioscience, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden 2) Cellectricon AB, Fabriksgatan 7, SE-412 50 Gothenburg, Sweden 3) Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260

Published in: Anal. Chem. 2005, 77, 4667-4672

Fish Eggs Spawn a DNA Delivery Revolution – Caviar inspires a Berkeley bioengineer to take electroporation to the single-cell level Author(s):

Laura lane

Published in: The Scientist 19 (13):34 (4 July 2005)

Electroporation and the Single Cell, New technologies increase the resolution of electroporation Author(s):

Aileen Constans

Published in: The Scientist 18 (8):46 (26 April 2004)

Single-cell electroporation Author(s):

Jessica Olofsson, Kerstin Nolkrantz, Frida Ryttsén, Bradley A. Lambie, Stephen G. Weber, Owe Orwar

Published in: Current Opinion in Biotechnology; 14(1), 29-34 (2003)

Functional Screening of Intracellular Proteins in Single Cells and in Patterned Cell Arrays Using Electroporation Author(s):

Kerstin Nolkrantz, Cecilia Farre, K. Johan Hurtig, Petra Rylander, Owe Orwar

Published in: Anal. Chem. 2002, 74, 4300-4305

Electroporation of Single Cells and Tissues with an Electrolyte-filled Capillary Author(s):

K. Nolkrantz, C. Farre, A. Brederlau, R.I.D. Karlsson, C. Brennan, P.S. Eriksson, S.G. Weber, M. Sandberg, O. Orwar

Published in: Anal. Chem., Sept 15, 73(18), 4469-4477 (2001)

Dynaflow

A method for bidirectional solution exchange – “Liquid bullet” applications of acetylcholine to a7 nicotinic receptors Author(s):

Nikolai Fedorov (1), Lisa Benson (1), John D. Graef (1), Jeremy Hyman (1), Jill Sollenberger (1), Fredrik Pettersson (2), Patrick M. Lippiello (1), Merouane Bencherif (1)

1. Targacept, Inc., Preclinical Research Department, 200 East First Street, Suite #300, Winston Salem, NC 27101, USA
2. Veprox AB, Hängpilsgatan 6, SE-426 77 Västra Frölunda, Sweden

Published in: Journal of Neuroscience Methods, Volume 206, Issue 1, 30 April 2012, Pages 23–33

Alternatively spliced domains interact to regulate BK potassium channel gating Author(s):

Brandon E. Johnson (a), Dominique A. Glausern (a), Elise S. Dan-Glauser (b), D. Brent Halling (c), Richard W. Aldrich (c) and Miriam B. Goodman (a).

a) Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305;
b) Department of Psychology, Stanford University, Stanford, CA 94305
c) Section of Neurobiology, Center for Learning and Memory, University of Texas, Austin, TX 78712

Published in: PNAS December 20, 2011 vol. 108 no. 51 20784-20789

Arrangement of Kv1 alpha subunits dictates sensitivity to tetraethylammonium Author(s):

Al-Sabi A, Shamotienko O, Dhochartaigh SN, Muniyappa N, Le Berre M, Shaban H, Wang J, Sack JT, Dolly JO.

International Centre for Neurotherapeutics, Dublin City University, Dublin 9, Ireland.

Published in: J Gen Physiol. 2010 Sep;136(3):273-82.

Novel Alpha-7 Nicotinic Acetylcholine Receptor Agonists Containing a Urea Moiety: Identification and Characterization of the Potent, Selective, and Orally Efficacious Agonist 1-[6-(4-Fluorophenyl)pyridin-3-yl]-3-(4-piperidin-1-ylbutyl) Urea (SEN34625/WYE-103914) Author(s):

Chiara Ghiron(1) et al;

(1) Siena Biotech SpA, Strada del Petriccio e Belriguardo 35, 53100 Siena, Italy
(2) Chemical Sciences and Neuroscience Discovery Research, Wyeth Research, CN 8000, Princeton, New Jersey 08543-8000

Published in: J. Med. Chem., 2010, 53 (11), pp 4379–4389 DOI: 10.1021/jm901692q

Telithromycin blocks neuromuscular transmission and inhibits nAChR currents in vitro Author(s):

Chang-Ning Liu, Chris J. Somps

Department of Investigative Toxicology, Drug Safety Research & Development, Pfizer Global R&D, Groton, CT 06340, USA

Published in: Toxicol Lett. 2010 May 4;194(3):66-9. Epub 2010 Feb 12.

MEC-2 and MEC-6 in the Caenorhabditis elegans Sensory Mechanotransduction Complex: Auxiliary Subunits that Enable Channel Activity Author(s):

Austin L. Brown(1), Zhiwen Liao(2), and Miriam B. Goodman(1,2)
1) Biophysics Program and 2) Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305

Published in: J. Gen. Physiol., Jun 2008; 131: 605 - 616.

Differential Structure of Atrial and Ventricular KATP Atrial KATP Channels Require SUR1 Author(s):

Thomas P. Flagg(1); Harley T. Kurata(1); Ricard Masia(1); George Caputa(1); Mark A. Magnuson(2); David J. Lefer(3); William A. Coetzee(4); and Colin G. Nichols(1)
1) Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Mo; Department of Molecular Physiology 2) Biophysics, Vanderbilt University School of Medicine, Nashville, Tenn 3) Department of Medicine and Pathology, Albert Einstein College of Medicine, New York; 4) Department of Pediatrics, New York University School of Medicine.

Published in: Circulation Research. 2008 Published online before print October 30, 2008, doi: 10.1161/CIRCRESAHA.108.17818

Role of cAMP sensor Epac as a determinant of K-ATP channel ATP-sensitivity in human pancreatic beta cells and rat INS-1 cells Author(s):

Guoxin Kang, Colin A Leech, Oleg G Chepurny, William A Coetzee, and George G Holz
New York University School of Medicine

Published in: Kang et al. J Physiol.2008; 0: jphysiol.2007.143818v1

Gain-of-Function Mutations in the MEC-4 DEG/ENaC Sensory Mechanotransduction Channel Alter Gating and Drug Blockade Author(s):

Austin L. Brown(1), Silvia M. Fernandez-Illescas(2), Zhiwen Liao2, and Miriam B. Goodman(1,2)
1) Biophysics Program and 2) Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305

Published in: J. Gen. Physiol., Jan 2007; 129: 161 - 173.

Controlling Desensitized States in Ligand-Receptor Interaction Studies with Cyclic Scanning Patch-Clamp Protocols Author(s):

Daniel Granfeldt(1), Jon Sinclair(2), Maria Millingen(1), Cecilia Farre(2), Per Lincoln(1) and Owe Orwar(1)
1) Department of Chemical and Biological Engineering, Physical Chemistry, Chalmers University of Technology, SE-412 96 Göteborg, Sweden 2) Cellectricon AB, Fabriksgatan 7, SE-412 50 Göteborg, Sweden

Published in: Anal. Chem.; 2006; ASAP Article; DOI: 10.1021/ac060812z

A Biohybrid Dynamic Random Access Memory Author(s):

Jon Sinclair(1), Daniel Granfeldt(1), Johan Pihl(2), Maria Millingen(1), Per Lincoln(1), Cecilia Farre(2), Lena Peterson(3), and Owe Orwar(1)
1) Department of Chemistry and Bioscience and Microtechnology Centre, Chalmers University of Technology, SE-412 96 Göteborg, Sweden 2) Cellectricon AB, Fabriksgatan 7, SE-412 50 Göteborg, Sweden 3) Department of Signal and Systems, Chalmers University of Technology, SE-412 96 Göteborg, Sweden

Published in: J. Am. Chem. Soc.; 128(15),5109-5113 (doi:10.1021/ja0580993), (2006)

An isoflurane- and alcohol-insensitive mutant GABAA receptor 1 subunit with near normal apparent affinity for GABA: characterization in heterologous systems and production of knock-in mice. Author(s):

Cecilia M. Borghese (1), David F. Werner (2), Norbert Topf (3), Nicole V. Baron (3), L. Ashley Henderson (4), Stephen L. Boehm II (5), Yuri A. Blednov (4), Abdallah Saad (6), Shuiping Dai (6), Robert A. Pearce (6), R. Adron Harris (1), Gregg E. Homanics (2), Neil L. Harrison(3)
1) University of Texas – Austin 2) University of Pittsburgh 3) Weill Medical College of Cornell University 4) University of Texas at Austin 5) State University of New York – Binghamton 6) University of Wisconsin – Madison

Published in: J. Pharmacol. Exp. Ther. 2006: jpet.106.104406v1

Blocking Characteristics of hERG, hNav1.5, and hKvLQT1/ hminK after Administration of the Novel Anti-Arrhythmic Compound AZD7009 Author(s):

Frida Persson, M.Sc.,Leif Carlsson, Ph.D., Göran Duker, Ph.D., and Ingemar Jacobson, Ph.D.
AstraZeneca R&D Mölndal, Mölndal, Sweden

Published in: J Cardiovasc Electrophysiol, 16(3), 329-341,(2005)

Microfluidic device for creating gradients Author(s):

Rajendrani Mukhopadhyay

Published in: Anal. Chem. A-Pages ; 2005; 77(13); 245A-245A.

A chemical waveform synthesizer Author(s):

Jessica Olofsson(1), Helen Bridle(1), Jon Sinclair(1), Daniel Granfeldt(1), Eskil Sahlin(2), and Owe Orwar(1)
1) Department of Chemistry and Bioscience and Microtechnology Centre, Chalmers University of Technology, SE-412 96 Göteborg, Sweden 2) Department of Chemistry, Göteborg University, SE-412 96 Göteborg, Sweden
Edited by Richard N. Zare, Stanford University, Stanford, CA

Published in: PNAS; 102(23), 8097-8102, (doi:10.1073/pnas.0500230102), (2005)

Microfluidic technologies in drug discovery Author(s):

Johan Pihl, Mattias Karlsson, Daniel T. Chiu

Published in: Drug Discovery Today, Vol 10, Number 20, October 2005

Patch clamp electrophysiology steps up a gear Author(s):

Derek J. Trezise, Assay & Compound Profiling, GlaxoSmithKline Research & Development

Published in: European Pharmaceutical review, Issue 2, 2005

Microfluidic Gradient Generating Device for Pharmacological Profiling Author(s):

Johan Pihl(1), Jon Sinclair(1), Eskil Sahlin(2), Mattias Karlsson(3), Fredrik Petterson(3), Jessica Olofsson(1), and Owe Orwar(1)
1) Department of Chemistry and Bioscience, and Microtechnology Centre, Chalmers University of Technology, SE-412 96 Göteborg, Sweden 2) Department of Chemistry, Göteborg University, SE-412 96 Göteborg, Sweden 3) Cellectricon AB, Fabriksgatan 7 SE-412 50 Göteborg, Sweden

Published in: Analytical Chemistry; 77(13), 3897-3903, (doi:10.1021/ac050218+), (2005)

Technology Feature: Cell biology, Maximizing return. Ion channels, stem cells and cell signalling are the focus of intense interest in both cell biology and drug discovery. Pete Moore takes a look at what’s on offer for the researcher. Author(s):

Pete Moore

Published in: Nature, Dec 1, 439 (6817), p697-699 (2005). doi:10.1038/438699b

Blocking characteristics of hKv1.5 and hKv4.3/hKChIP2.2 after administration of the novel antiarrhythmic compound AZD7009 Author(s):

Persson, Frida MSc; Carlsson, Leif PhD; Duker, Goran PhD; Ingemar Jacobson PhD
AstraZeneca R&D Mölndal, Mölndal, Sweden

Published in: Journal of Cardiovascular Pharmacology 46(1): 7-17. (2005).

Microfluidics and Patch-Clamp Based Sensors Author(s):

Cecilia Farre, Mattias Karlsson, Jon Sinclair

Published in: Genetic Engineering News, Vol. 24, No 21, December 2004

A Microfluidics Approach to the Problem of Creating Separate Solution Environments Accessible from Macroscopic Volumes Author(s):

Jessica Olofsson(1),Johan Pihl(1),Jon Sinclair(3),Eskil Sahlin(2), Mattias Karlsson(2), and Owe Orwar(1)
1)Department of Chemistry and Bioscience, and Microtechnology Centre, Chalmers University of Technology, SE-412 96 Göteborg, Sweden, 2) Cellectricon AB, Fabriksgatan 7 SE-412 50 Göteborg, Sweden, and 3) Department of Chemistry, Gothenburg University, SE-412 96 Göteborg, Sweden

Published in: Analytical Chemistry; 76(17), 4968 - 4976, (doi:10.1021/ac035527j), (2004)

Stabilization of High-Resistance Seals in Patch-Clamp Recordings by Laminar Flow Author(s):

Jon Sinclair, Jessica Olofsson, Johan Pihl, and Owe Orwar
Department of Physical Chemistry and Microtechnology Centre, Chalmers University of Technology, SE-412 96 Göteborg, Sweden

Published in: Analytical Chemistry; 75(23), 6718-6722, (2003), (Technical Note)

Automated Electrophysiology: High Throughput of Art Author(s):

Xiaobo Wang, Min Li

Published in: ASSAY and Drug Development Technologies. Oct 2003, Vol. 1, No. 5: 695-708

A Cell-Based Bar Code Reader for High-Throughput Screening of Ion Channel-Ligand Interactions Author(s):

Jon Sinclair(1),Johan Pihl(1), Jessica Olofsson(1), Mattias Karlsson(1), Kent Jardemark(3), Daniel T. Chiu(2), and Owe Orwar(1)
1) Department of Physical Chemistry, and Microtechnology Centre, Chalmers University of Technology, SE-412 96 Göteborg, Sweden 2) Department of Chemistry, University of Washington, Seattle, Washington 98195-1700 3) Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden

Published in: Analytical Chemistry; Dec 15, 74(24), 6133-6138 (2002)

Screening of ion channel receptor agonists using capillary electrophoresis-patch clamp detection with resensitized detector cells Author(s):

Cecilia Farre(1), Andreas Sjöberg(1), Kent Jardemark(1), Ingemar Jacobson(2), and Owe Orwar(1)
Department of Chemistry, Göteborg University, SE-412 96 Göteborg, Sweden, and AstraZeneca AB, SE-431 83 Mölndal, Sweden

Published in: Anal. Chem., Mar 15, 73(6), 1228-33 (2001)

Cellectrion Related

Life Science Technologies Microfluidics—Bringing New Things to Life Science Author(s):

Jeffrey M. Perkel

Published in: AAAS/Science, 6 November 2008

Cellectricon: Insights from inside Author(s):

Ludger Wess

Published in:

Selective introduction of antisense oligonucleotides into single ***** CNS progenitor cells using electroporation demonstrates the requirement of STAT3 activation for CNTF-induced gliogenesis Author(s):

Åberg MA, Ryttsen F, Hellgren G, Lindell K, Rosengren LE, MacLennan AJ, Carlsson B, Orwar O, Eriksson PS.

Published in: Mol. Cell. Neurosci., Mar, 17(3), 426-43 (2001)

Chemical transformations in individual ultrasmall biomimetic containers Author(s):

D. T. Chiu, C. F. Wilson, F. Ryttsén, A. Strömberg, C. Farre, A. Karlsson, S. Nordholm, A. Gaggar, B. P. Modi, A. Moscho, R. A. Garza-López, O. Orwar, R. N. Zare

Published in:

Electroinjection of colloid particles and biopolymers into single unilamellar liposomes and cells for bioanalytical applicatons Author(s):

M. Karlsson, K. Nolkrantz, M. J. Davidson, A. Strömberg, F. Ryttsén, B. Åkerman, O. Orwar

Published in: Anal. Chem., 72(23), 5857-5862 (2000)

Manipulating the Genetic Identity and Biochemical Surface Properties of Individual Cells with Electric-Field-Induced Fusion Author(s):

A. Strömberg, F. Ryttsén, D. T. Chiu, M. Davidson, P. S. Eriksson, C. F. Wilson, O. Orwar, R. N. Zare

Published in: PNAS, 97(1), 7-11(2000)

Characterization of single-cell electroporation by using patch-clamp and fluorescence microscopy Author(s):

Ryttsen F, Farre C, Brennan C, Weber SG, Nolkrantz K, Jardemark K, Chiu DT, Orwar O.

Published in: Biophys. J., Oct; 79(4): 1993-2001 (2000)

Altering the Biochemical State of Individual Cultured Cells and Organelles with Ultramicroelectrodes Author(s):

J. A. Lundqvist, F. Sahlin, M. A. I. Åberg, A. Strömberg, P. S. Eriksson, O. Orwar

Published in: PNAS, 95, 10356-10360 (1998)