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主营产品: Flexcell细胞力学和regenhu细胞3D生物打印机销售技术服务: 美国Flexcell品牌FX-5000T细胞牵张应力加载培养系统,FX-5K细胞显微牵张应力加载培养系统,Tissue Train三维细胞组织培养与测试系统,FX-5000C三维细胞组织压应力加载培养系统,STR-4000细胞流体剪切应力加载培养系统,德国cellastix品牌Optical Stretcher高通量单细胞牵引应变与分析系统 Regenhu品牌3D discovery细胞友好型3D生物打印机,piuma细胞纳米压痕测试分析、aresis多点力学测试光镊,MagneTherm细胞肿瘤电磁热疗测试分析系统
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Gel Microdrop (GMD) Secretion Assay

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  • 产品名称:Gel Microdrop (GMD) Secretion Assay
  • 产品型号:GMD
  • 产品展商:onecell
  • 产品文档:无相关文档
简单介绍

The Gel Microdrop (GMD) Secretion Assay rapidly identifies cells that secrete a protein of interest. Viable, high-secreting or rare cells can be isolated and recovered using fluorescence activated cell sorting (FACS). Please click here for more information on the GMD Protein Secretion Assay and FACS. Encapsulated cells are analyzed or recovered using flow cytometry based on the amount of p

产品描述
The Gel Microdrop (GMD) Secretion Assay rapidly identifies cells that secrete a protein of interest. Viable, high-secreting or rare cells can be isolated and recovered using fluorescence activated cell sorting (FACS). Please click here for more information on the GMD Protein Secretion Assay and FACS.
 
  Encapsulated cells are analyzed or recovered using flow cytometry based on the amount of protein captured in each GMD. The capture and detection is analogous to the sandwich antibody format used in enzyme-linked immunosorbent assays (ELISA), however, the immunoreaction itself takes place within the agarose microsphere, leaving the encapsulated cell intact. A schematic representation of the GMD secretion assay is depicted below.
 
 
 
 

The GMD Secretion Assay Involves Four Easy Steps


 
 


 
  Protein secreted by an individual cell is captured and fluorescently labeled within the GMD. Unoccupied GMDs and GMDs occupied by non-secreting cells do not fluoresce. The fluorescence intensity of each occupied GMD corresponds to the amount of protein secreted by the cell. Fluorescing GMDs occupied by secreting cells can be sorted and recovered based on level of protein secretion.  
 

Advantages of the GMD Protein Secretion Assay


 
  We offer a lease program which includes a CellSys 100™ Microdrop Maker, CelMix™ and CelBioGel™ encapsulation reagents, training, and technical support. Alternatively, if you have a characterized cell line and two non-overlapping antibodies (one biotinylated and one fluorescently labeled), we will screen and sort your cells on a fee-for-service basis. Please contact us for details regarding either of these arrangements.  
       
 

Secretion Assay Forms And Additional Information (click on the PDF to view)

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