为纳米技术研究市场提供光学图像处理的CytoViva公司将一种新型光谱图像技术（HSI）引入到**纳米显微镜系统中。这种技术的结合将帮助科学家在纳米医学和纳米材料研究取得重大进展。 在过去的几年里，CytoViva的“纳米显微镜系统"被很多****纳米技术研究机构采纳，包括世界研究医院，约翰霍普金斯医学院、德州安德森癌症中心、美国FDA、NASA，NIH等。这一技术受到广泛好评，并获得两项RD100大奖，一项 Nano50 大奖。
CytoViva™纳米荧光高光谱显微成像系统(HSI)，可以同时提供纳米材料及生物样品的光谱分析和图像数据。该系统在可见-近红外光谱范围内（VNIR）进行数据采集，提供定量及定性分析数据，对活体细胞和纳米材料的荧光或非荧光成分适用。
双模式荧光显微成像系统：支持荧光标记和非荧光标记物质同时观察；支持无机物质如：liposome，环碳纳米管，及其它纳米粒子观察和组织切片、细胞的荧光/非荧光成像；支持光谱分析等功能。
CytoViva™是一种应用在生物医学和纳米技术研究领域的紧凑，超高对比度，超高分辨率，实时光学显微镜。该CytoViva™的光学照明系统是****的，取代了标准的显微镜聚光器，并提供优化的光学分辨率和检测。科学家们可以快速，方便地观察各种各样的纳米材料。观察到在传统光学成像技术无法观测到的，活细胞和**的细节。通过将CytoViva双模荧光系统，研究人员还可以观察到荧光标记的纳米颗粒，与无标记的**和活细胞的相互作用。这种独特的技术可以无需计算机的特殊处理，只需两种不同的照明方式和**的软件程序。
CytoViva™是一种**性的产品，能够创造新的荧光和高分辨率结合的光学显微成像，达到****的水平。这种独特的系统使研究人员能够实时的，高分辨率的同时观察两个荧光和非荧光样品的结构。拥有CytoViva™，研究人员现在就可以在自己的实验室，随时动态观察亚100nm的纳米材料和生物活体标本的高分辨率成像。
CytoViva™将您现有的研究显微镜的性能提升到一个新的水平。
实时观察：
· 活细胞和纳米材料的亚100纳米分辨率
· 同时荧光和非荧光样品结构
纳米高光谱显微成像系统：高光谱成像系统配合纳米显微成像系统，可以广泛应用于量化细胞和组织中的纳米材料。该系统捕获扫描范围内近红外（400-1000nm）内每个像素的光谱信号；每一像素的完整光谱被CCD采集，光谱分辨率高达2nm，这些数据同时以光谱曲线和RGB图像形式呈现，每一个视野的详细定量分析可以被执行，**的分析软件可以提供的扫描材料的详细光谱信息。适用于纳米**输送和纳米毒理学领域。 此高光谱分析方法既支持活细胞和纳米材料的非荧光标记，也支持荧光标记。
虽然这个系统是专门设计用于支持纳米级的研究，同时它也支持一个广泛的，如细胞生物学和传染性**领域的生物医学研究。
Dr. Carlos Lopez-Estrano : University of Memphis College of Medicine
Dr. John Elliott: NIST
纳米荧光高光谱成像
下面的图片是CytoViva™系统在纳米生物研究领域有代表性的一个典型应用。此文章说明了CytoViva™系统对100nm的纳米金颗粒，和一个活上皮细胞结合能力的量化。
技术参数：
应用领域：
纳米材料、纳米医药、纳米**递送、纳米毒理学、细胞生物学、病理学、病毒学、植物学......
英文介绍
The CytoViva® Microscope System
Specifically designed to support research in nanotechnology and infectious disease, CytoViva employs a patented (US patents No. 7,542,203, 7,564,623) darkfield-based optical illumination system. This structured illumination technology replaces the standard condenser on a research grade microscope. By improving the alignment and focus of darkfield or oblique angle illumination, the technology enhances signal-to-noise of nanoscale samples up to seven times over standard darkfield optics. This enables scientists to optically observe a wide range of nanoscale materials quickly and easily in solution, live cells, tissue and materials based matrices. In addition, non-fluorescent live cells and pathogens can be easily observed at a level of detail not possible with traditional optical imaging techniques such as phase contrast or differential interference contrast.
When using the CytoViva Dual Mode Fluorescence system, researchers can also observe the interactions between fluorescently labeled nano-particles or bacteria and live unlabeled cells. This unique capability can eliminate the need to create computer enhanced overlay images which require two different illumination methods and advanced software programs. Finally, when combined with CytoViva’s Hyperspectral Imaging capability this high signal-to-noise microscopy method enables researchers to spectrally characterize and map nanoscale samples in a wide range of environments.
用户评价：
J. Paul Robinson, PhD, Director of Purdue University's Cytometry Laboratory, Professor of Basic Medical Sciences and Biomedical Engineering, Purdue University comments...
""It's like not knowing you need glasses. You don't know what you can't see. Then someone hands you a pair-and the world is clear with amazing detail.""
John A. Smith, MD, PhD, MMM, Divisional Director, Department of Pathology, University of Alabama-Birmingham says it well ...
""Looking through CytoViva, you are face to face with living biology. You see into the world of cell biology
that you didn't know existed. You are visualizing the future of underlying biological processes as it merg
es with the present.""
Dr. Elaine Coleman, Associate Professor, Auburn University, Department of Anatomy, Physiology and Pharmacology comments...
""Its capabilities in cell culture research are astounding. I have purchased a unit for my future research because its capabilities are unique for observing live cell cultures.""
部分用户：
National Institute for Occupational Health
US Food and Drug Administration
Finnish Institute for Occupational Health
National Institute of Health Sciences Japan
Melbourne Center for Nanofabrication
US Army Corp of Engineers
Lawrence Berkeley National Labs
IIT Madras
Rice University
Fraunhofer Institute
Adolph Merkle Institute
University of Sao Paulo
Health Canada
Stanford University
University of Bonn
Georgia Tech University
Duke University
University of Montreal
Wright Patterson AFB
MD Anderson Cancer Center
Center for Nanoscale Science and Engineering
文献：
有超过300篇用CytoViva™纳米荧光高光谱显微成像系统发表的第三方文献，这是*近发表的部分文献：
Hyperspectral imaging for cellular iron mapping in the in vitro model of Parkinson's disease
Eung Seok Oh, Chaejeong Heo, Ji Seon Kim, Minah Suh, Young Hee Lee, Jong-Min Kim
Journal of Biomedical Optics | Volume 19 | Issue 5 | Special Section on Nanobio-Based Optical Sensing and Imaging 2014
Optical hyperspectral investigation of the J-pole and Vee antenna families
Timothy D. James, Timothy J. Davis, and Ann Roberts
Optics Express, Vol. 22, Issue 2, pp. 1336-1341 (2014)
In Vitro Identification of Gold Nanorods through Hyperspectral Imaging
Bradley M. Stacy, Kristen K. Comfort, Donald A. Comfort, Saber M. Hussain
Plasmonics June 2013, Volume 8, Issue 2, pp 1235-1240
Hyperspectral enhanced dark field microscopy for imaging blood cells
Giulia Sacco Verebes, Michele Melchiorre2, Adianez Garcia-Leis, Carla Ferreri, Carla Marzetti,
Armida Torreggiani
Journal of Biophotonics Volume 6, Issue 11-12, pages 960–967, December 2013