Ø 应用于透性 (去膜) 心肌细胞的研究
Ø 测试kTR, 力-钙离子浓度, 长度-张力, 力-收缩速率和硬度等机械特性
Ø 备有8个溶液小槽的寛阔平台以盛载不同浓度的钙离子溶液
Ø 电动化三维微定位装置以准确和快速地将样本定位
Ø 可以配合标准显微镜或者倒置显微镜
Ø 可控温度范围: 0-40°C
1600A 是一个可靠和可重复性的实验测试系统,应用于测试去膜心肌细胞的主动和被动性质。透过使用其摇杆控制电动化定位装置可以轻易地令细胞的两端分别连接于力传感器和高速伺服电机,这大大提高了实验效能和简化了原本精细的样本连接程序。
可控温浴盆拥有8个溶液小槽令细胞可以轻易地在不同浓度的钙离子溶液之间进行转换,该装置会直接放置在倒置显微镜的置物平台上进行高清晰度的观察和肌节间距的检测。可选择附加一块棱镜去准确地检测出细胞于溶液槽的深度。
1400A测试系统可以控制和测试力度, 长度和肌小节的间隔长度(当使用可附加的高速视频肌节长度系统) ,使其应用范围更广泛, 从简单的力-钙离子浓度曲线去到样本完整的机械特性(例如kTR, 长度-张力, 力-收缩速率和硬度) 都能够被测试。附加的软件程序(600A) 备有各种标准的实验方案去配合不同的实验需求。
完整系统包括:
●高速长度操控仪
● 400A力传感器
●电动化三维微定位平台
●带有8个溶液小槽的可控温平台
●客制桌用电脑(附加萤幕): 配有控制和分析软件系统
●温度控制器和冷却液循环器
●可附加肌小节间隔长度检测软件(HVSL/ VSL)
应用文献:
一、心肌细胞和肌肉文献
American Journal of …, 2005 - Am Physiological Soc
... Power output is linearly related to MyHC content in rat skinned myocytes and isolated working
hearts. ... were made using a capacitance-gauge transducer (model 403; sensitivity, 20 mV/mg plus
a x10 amplifier; resonant frequency, 600 Hz; Aurora Scientific; Aurora, Ontario ...
Cited by 45 - Related articles - Get at CISTI - All 5 versions
Altered single cell force-velocity and power properties in exercise-trained rat myocardium
[HTML] from physiology.org
GM Diffee… - Journal of Applied Physiology, 2003 - Am Physiological Soc
... The resulting skinned myocytes were resuspended in 8–10 ml of relaxing solution and kept on
ice throughout the days ... described previously (8). Skinned cardiac myocyte preparations were
attached between a capacitance-gauge transducer (model 403, Aurora Scientific) and a ...
Cited by 25 - Related articles - Get at CISTI - All 8 versions
Regional differences in effects of exercise training on contractile and biochemical properties of rat cardiac
myocytes
[HTML] from physiology.org
GM Diffee… - Journal of Applied Physiology, 2003 - Am Physiological Soc
... The resulting skinned myocytes were resuspended in 8–10 ml of relaxing solution and kept on
ice throughout the days ... described previously (9). Skinned cardiac myocyte preparations were
attached between a capacitance-gauge transducer (model 403, Aurora Scientific) and a ...
Cited by 24 - Related articles - Get at CISTI - All 5 versions
Force-length relations in isolated intact cardiomyocytes subjected to dynamic changes in mechanical load
[HTML] from physiology.org
G Iribe, M Helmes… - American Journal of Physiology- …, 2007 - Am Physiological Soc
... Gentaro Iribe, 1 Michiel Helmes, 1 ,2 and Peter Kohl 1. ... CCD, charge-coupled device. K was
measured directly, using a force transducer system (406A; Aurora Scientific, Aurora, ON, Canada)
factory calibrated for compression measurement, and mounted under a microscope. ...
Cited by 30 - Related articles - Get at CISTI - All 7 versions
Mouse intact cardiac myocyte mechanics: cross-bridge and titin-based stress in unactivated cells
[HTML] from rupress.org
…, N Harrell, CS Chung, M Helmes… - The Journal of …, 2011 - jgp.rupress.org
... Results were compared with those of previous studies on skinned myocytes (Helmes et al., 1996,
2003; Preetha et al., 2005) and ... fibers protruding from pipette tip), and their stiffness was determined
by cross-calibrating with a force transducer (model 406A; Aurora Scientific). ...
Cited by 5 - Related articles - All 6 versions
Calcium sensitivity and the Frank-Starling mechanism of the heart are increased in titin N2B region-deficient mice
[HTML] from nih.gov
EJ Lee, J Peng, M Radke, M Gotthardt… - Journal of molecular and …, 2010 - Elsevier
... papillary muscles were dissected into small strips (cross-sectional area (CSA) 0.02 mm 2 ; length
1.2 mm) and small aluminum clips were glued to the ends of the muscle [33] in order to attach
the muscle at one end to a force transducer (model 406, Aurora Scientific) and at the ...
Cited by 5 - Related articles - All 6 versions
Analysing force–pCa curves
[HTML] from nih.gov
JS Walker, X Li… - Journal of muscle research and cell motility, 2010 - Springer
... Department of Medicine, University of Colorado Denver, Aurora, CO 80045, USA e-mail:
john.s.walker@ucdenver.edu ... contrast ability at magnifications up to 400 9 . Sarcomere length
was measured for each myocyte using a image based system (Aurora Scientific 901A HSVL ...
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Mouse intact cardiac myocyte mechanics: cross-bridge and titin-based stress in unactivated cells
[HTML] from rupress.org
NMP King, M Methawasin, J Nedrud… - The Journal of …, 2011 - jgp.rupress.org
... Next, 10 ml of myocyte stopping buffer 2 was added (perfusion buffer plus 0.05 ml BCS/ml buffer
and 12.5 μM CaCl 2 ... mm of carbon fibers protruding from pipette tip), and their stiffness was
determined by cross-calibrating with a force transducer (model 406A; Aurora Scientific). ...
Cited by 5 - Related articles - All 6 versions
Inorganic phosphate speeds loaded shortening in rat skinned cardiac myocytes
[HTML] from physiology.org
AC Hinken… - American Journal of Physiology- …, 2004 - Am Physiological Soc
... were introduced at one end of the preparation with a direct current (DC) torque motor (model
308; Aurora Scientific) driven by ... window In a new window. Table 2. Effects of P i addition on
mechanical properties of skinned cardiac myocyte preparations. ... 2. Araujo A and Walker JW. ...
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Protein Kinase A–Mediated Acceleration of the Stretch Activation Response in Murine Skinned Myocardium Is
Eliminated by Ablation of cMyBP-C
[HTML] from ahajournals.org
JE Stelzer, JR Patel… - Circulation research, 2006 - Am Heart Assoc
... The ends of the preparation were attached to the arms of a motor (model 312B, Aurora Scientific)
and force transducer (model 403, Aurora Scientific), as described earlier. ... Circ Res. 1998; 83:
471–480. Abstract/FREE Full Text. ↵ de Tombe PP, Stienen GJM. ...
Cited by 41 - Related articles - Get at CISTI - All 7 versions
Cycling cross-bridges increase myocardial stiffness at submaximal levels of Ca2+ activation
[HTML] from nih.gov
KS Campbell, JR Patel… - Biophysical journal, 2003 - Elsevier
... 913 ± 53 μm, width 130 ± 12 μm) were connected between a motor (Model 312B, time for
100-μm step 1 ms, Aurora Scientific, Ontario, Canada ... Many previous reports (Noble, 1977; Chiu
et al., 1982; de Tombe and ter Keurs, 1992; [Stuyvers et al., 1997] and [Stuyvers et al., 1998 ...
Cited by 26 - Related articles - Get at CISTI - All 11 versions
Effect of stimulation rate, sarcomere length and Ca2+ on force generation by mouse cardiac muscle
[HTML] from physoc.org
BD Stuyvers, AD McCulloch, J Guo… - The Journal of …, 2002 - Physiological Soc
... The length of the muscle (ML) was set using a servo-controlled motor (model 318 B, Aurora
Scientific Inc., Aurora, ON, Canada) hooked to ... carbon fibre rod, constituted the force transducer
so that the resolution of the force measurement system was 0.5 μN (see Stuyvers et al. ...
Cited by 45 - Related articles - Get at CISTI - All 10 versions
Effects of thin and thick filament proteins on calcium binding and exchange with cardiac troponin C
[HTML] from nih.gov
JP Davis, C Norman, T Kobayashi, RJ Solaro… - Biophysical journal, 2007 - Elsevier
... Aluminum T-clips were used to mount the trabecula between the arms of a high-speed
length controller (model 322C, Aurora Scientific, Ontario, Canada) and an isometric force
transducer (model 403A, Aurora Scientific) in relaxing solution. ...
Cited by 39 - Related articles - Get at CISTI - All 8 versions
Fabrication and Evaluation of Reconstructed Cardiac Tissue and Its Application to Bio-actuated Microdevices
H Horiguchi, K Imagawa, T Hoshino… - NanoBioscience, …, 2009 - ieeexplore.ieee.org
... D. Contractile Force Measurement of the Reconstructed Cardiac Tissue The contractile force of
the reconstructed cardiac tissue was measured using a force transducer. One end of the tissue
was fixed to a permeabilized fiber test apparatus (803B, Aurora Page 3. ...
Cited by 3 - Related articles - All 4 versions
The mechanoelectric feedback: a novel “calcium clamp” method, using tetanic contraction, for testing the role of
the intracellular free calcium
Y Yaniv, C Levy… - … of the New York Academy of …, 2006 - Wiley Online Library
... 308B, Aurora Scientific, Richmond Hill, Ontario, Canada) with a rise time of 250 μsec. ... from the
rat right ventricle (Spargue Dawley, 250–300 g). The dissection and the technique of mounting
the trabeculae in the experiment setup followed the procedures developed by ter Keurs. ...
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Length dependence of force generation exhibit similarities between rat cardiac myocytes and skeletal muscle
fibres
[HTML] from physoc.org
LM Hanft… - The Journal of Physiology, 2010 - Wiley Online Library
... Length changes were introduced using a DC torque motor (model 308, Aurora Scientific, Inc.)
driven by voltage commands from a ... The kinetics of force redevelopment were obtained using
a procedure previously described for skinned cardiac myocyte preparations (Korte et al. ...
二、活体动物肌肉机械特性测试文献
Plasmid-based gene transfer in mouse skeletal muscle by electroporation
JD Schertzer, GS Lynch - METHODS IN MOLECULAR BIOLOGY- …, 2008 - Springer
... 1. The measurement of TA muscle function in situ requires a suitable platform (which is usually
Plexiglas) to secure the anesthetized mouse and for appropriate positioning of the servomotor. ...
Suitable systems are commer- cially available (Aurora Scientific, Aurora, ON, Canada). ...
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[HTML] < em> In Vivo</em> Canine Muscle Function Assay
[HTML] from jove.com
MK Childers, RW Grange… - Journal of Visualized …, 2011 - jove.com
... Stimulator, Grass, S88X, Stimulus isolation unit, Grass, SIU-C, Muscle lever system, Aurora
Scientific, 310C-LR, Footplate option. Signal interface, Aurora Scientific, Series 604A, Dynamic
Muscle Control & Analysis Software, Aurora Scientific, Aurora Scientific, ...
Cited by 1 - Related articles - Cached - All 4 versions
Monitoring Murine Skeletal Muscle Function for Muscle Gene Therapy
[HTML] from nih.gov
CH Hakim, D Li, D Duan, CH Hakim, D Li… - Methods in molecular …, 2011 - Springer
... 1. Physiological saline (0.9% NaCl). 2. In situ muscle function assay system (Aurora
Scientific). The system consists of a 305C-LR dual mode transducer, a 701B stimulator,
a 604A signal interface, and an 809A footplate apparatus (Fig. 1c). ...
Androgen replacement therapy improves function in male rat muscles independently of hypertrophy and
activation of the Akt/mTOR pathway
C Hourde, C Jagerschmidt… - Acta …, 2009 - Wiley Online Library
... The right limbs were fixed with clamps (806A; Aurora Scientific, Aurora, Canada). The distal
tendons of the right soleus muscles were attached to a dual-mode lever arm system that measures
muscle isometric force (300C; Aurora Scientific) using a silk ligature. ...
Glutamine preserves skeletal muscle force during an inflammatory insult
BM Meador… - Muscle & nerve, 2009 - Wiley Online Library
... Force was measured using a dual-mode lever system, which controlled both length and
force/speed (305C-LR; Aurora Scientific). ... The ankle of the mouse was fixed in the servomotor's
footplate, with the talus (rotational center of the ankle joint) aligned with the servomotor axis. ...
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Sarcolipin overexpression in rat slow twitch muscle inhibits sarcoplasmic reticulum Ca2+ uptake and impairs
contractile function
[HTML] from jbc.org
AR Tupling, M Asahi… - Journal of Biological Chemistry, 2002 - ASBMB
... Three days after DNA injection, electrically evoked muscle force was assessed in situ from the
soleus muscle from each hindlimb across a range ... using a Grass S88 stimulator, and force data
were collected on-line using a 640A signal interface (Aurora Scientific Inc.) connected ...
Cited by 45 - Related articles - BL Direct - All 4 versions
Tibialis anterior muscles in mdx mice are highly susceptible to contraction-induced injury
[PDF] from 141.213.232.243
C Dellorusso, RW Crawford, JS Chamberlain… - Journal of muscle …, 2001 - Springer
... no. 85-23 (NIH), Revised 1985, Office of Science and Health reports, Bethesda, MD). In
situ preparation ... The tendon of the muscle was tied securely to the lever arm of a servomotor
(Model 305B-LR, Aurora Scientific, Richmond Hill, ON, Canada). ...