Flexflow单通道平行板流室系统提供流体切应力同时抻拉细胞
FlexFlow显微切应力加载设备（SHEAR Stress device）
流体剪切应力培养载片如下图：
细胞培养载片包括显微镜载(物)片和盖玻片两种产品，表面经过特殊处理，适合于细胞的贴壁与生长。
两种规格：75 mm x 25 mm x 1.0 mm ,75 mm x 24 mm x 0.2 mm 。
75 mm x 25 mm x 1.0 mm 细胞培养载片的边缘涂有1.0 mm宽的特氟隆边框(Teflon)，可以有效控制细胞生长在切应力加载区域。
自身荧光低，光学性能佳。
不同包被的培养表面提高细胞的贴壁与生长。
五种不同包被的培养表面：Amino, Collagen (Type I or IV) Elastin, ProNectin (RGD), Laminin (YIGSR).
所以产品都是无菌独立包装，仅供一次性使用。
75mm x 24mm x 0.2mm 和 FlexFlow配套使用
产品编号 英文名称
FFCS-U Culture Slips — Untreated
FFCS-A Culture Slips — Amino
FFCS-C Culture Slips — Collagen Type I
FFCS-C(IV) Culture Slips — Collagen Type IV
FFCS-E Culture Slips — Elastin
FFCS-P Culture Slips — ProNectin
FFCS-L Culture Slips — Laminin
3）微流纳流HiQ Flowmate微流体控制器
双注射泵可以在微升、纳升、微微升水平上控制液流.双注射泵，独立的液流控制系统。
传送**，稳定的流速
可控流速范围1.2pL/ min-260.6ml/min
提供不同流速模型：稳定型，脉冲型，连续型，截流型和震荡型；
可进行循环，连续的液流控制；同时运行不同的流速模型；
内置阀门控制液流模式；
机载计算器用于流量、流时、流速、剪切力的计算；
高分辨率、触屏控制。
用户友好的图标驱动程序；
便于泵和芯片对接的生物芯片支架；根据现有流速有三种不同的机型；
多种应用程序：
液体稀释，配给及注射器；
动物实验中的**注射和体液抽取；
施加液流剪切力；
微流体和纳流体实验；
混合、分流液体；
震荡型液流的控制需要iHIQ Flowmate二级阀门配件
系统完整结构图：
可以在提供流体切应力的同时抻拉细胞，测试血管和结绨组织细胞对液体流动的实时反应。
为培育在StageFlexer硅胶模表面或者基质蛋白包被的细胞培养片上的细胞提供切应力。
使用FX-5000T应力加载系统抻拉细胞，并且可以在实验前，实验中或者实验后提供切应力。
计算机控制蠕动泵，调节切应力大小，从0-35 dynes/cm2
使用标准正立式显微镜实时观察细胞在切应力下的反应。
检测细胞在流体作用下的排列反应。
加力同时实时检测在液体切应力下各种激活剂/抑制剂对细胞反应的影响。使用荧光团例如FURA-2检测细胞内[Ca2+]ic或者其它离子对切应力反应。（可以与str-4000六通道切应力系统配套使用）
FlexFlow系统包括：
FlexFlow装置；StreamSoft软件
FlexFlow快拆接头、胶管、FlexFlow 旁路连接器
MASTERFLEX L/S型号7550-10蠕动泵及配套线缆、连接管
2个稳流器；硅润滑剂
FX -5000 张力系统适配器
显微镜适应性FlexFlow底座
快速链接细胞培养基瓶；一个快速链接真空瓶
三个没**和六个**胶原蛋白涂层薄培养载片 （载片规格：75 mm x 24 mm x 0.2 mm）
三个没**和六个**胶原涂层StageFlexer膜
配件包
保证细胞在不同水平恒流或生理剪切力作用下仍保持黏附，在研究中得到了广泛应用。用蠕动泵（peristaltic pump）或注射泵（syringe pump）提供瞬态剪切力使平行板流室的入流管和出流管之间产生压差，使流室内细胞受到均匀，震荡或脉动剪切力的作用
微流纳流HiQ Flowmate微流体控制器
应用文献
FLEXFLOW™ AND STREAMER FLUID SHEAR STRESS SYSTEMS
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