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主营产品: Flexcell细胞力学和regenhu细胞3D生物打印机销售技术服务: 美国Flexcell品牌FX-5000T细胞牵张应力加载培养系统,FX-5K细胞显微牵张应力加载培养系统,Tissue Train三维细胞组织培养与测试系统,FX-5000C三维细胞组织压应力加载培养系统,STR-4000细胞流体剪切应力加载培养系统,德国cellastix品牌Optical Stretcher高通量单细胞牵引应变与分析系统 Regenhu品牌3D discovery细胞友好型3D生物打印机,piuma细胞纳米压痕测试分析、aresis多点力学测试光镊,MagneTherm细胞肿瘤电磁热疗测试分析系统
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VC-LFR-25-SS ,25毫米不锈钢圆形层流腔室,Laminar Flow Round, 25 mm, Stainless Steel

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  • 产品名称:VC-LFR-25-SS ,25毫米不锈钢圆形层流腔室,Laminar Flow Round, 25 mm, Stainless Steel
  • 产品型号:VC-LFR-25-SS
  • 产品展商:fluorescence
  • 产品文档:无相关文档
简单介绍

VC-LFR-25,VC-LFR-25-H型号圆形层流腔室,Laminar Flow Round, Models VC-LFR-25, VC-LFR-25-H; Laminar Flow Round, Models VC-LFR-18, VC-LFR-18-H

产品描述


 

Laminar Flow
Vacu-Cell
TM Incubation Chambers*
for 18 and 25 mm Round Coverslips

Flow Profile

LFR-25 Flow Profile

This series of incubation chambers all have the common feature of a laminar flow chamber on the underside of the chamber.  The arrows in the image to the left illustrate the direction of perfusion flow.  The standard fluidic ports for sample introduction or perfusion use 1/16 inch OD or 1/16 ID tubing, depending on the model type.  The narrow space between the o-rings is connect to a vacuum that function to hold the coverslip to the chamber body.  You can use theModel VCS-1 vacuum system provided by C&L Instruments or an ordinary laboratory vacuum.  

 

Laminar Flow Round, Models VC-LFR-25, VC-LFR-25-H

Model D48 Detector Module

Model VC-LFR-25

The Laminar Flow incubation perfusion chamber forms a small flow chamber using a coverslip or other flat surface.  The chamber body has a diameter of 1.12 inches and is 0.65 inches tall.  The coverslip is held to the chamber body by a vacuum. 

Dual o-rings isolate the vacuum from the area of the coverslip under study and securely hold the coverslip in place, forming a laminar perfusion chamber. 

The chambers made of metal (aluminum or stainless steel) are available with integrated heating elements for precise temperature control (Model VC-LFR-25-H). They can be fabricated with other materials on special request.

Although this Vacu-CellTM is designed for  a 25 mm coverslip, it will adhere to a larger surface.  It will form a chamber with virtually any flat surface having a minimum of a 25 mm diameter.  Shown on the left is the chamber adhered to the bottom of one well of a six well microtitre plate without the use of a coverslip. These chambers can be connected in series or in parallel to make a variety of incubation configurations. 

This chamber uses 1/16 OD tubing for the perfusion ports and 1/16 inch ID tubing for the hose barb vacuum port.  The chamber is sold as a kit that included the chamber, o-rings, connectors and tubing.

Laminar Flow Round, Models VC-LFR-18, VC-LFR-18-H

Model VC-LFR-18

We also offer a similar Laminar Flow design for use with 18 mm diameter coverslips.  For added convenience, the models LFR-25 and LFR-18 chambers have the same outer diameter (1.12 inches) and use the identical microscope chamber holder. 

Although this Vacu-CellTM is designed for an 18 mm coverslip, it will adhere to a larger surface.  It will form a chamber with virtually any flat surface having a minimum of an 18 mm diameter.  The dimensions of the flow chamber are listed in the table below.  size of the flow chamber is 4.8 mm (length) x 8.0 mm (width) x 0.25 mm (depth).

The chambers made of metal (aluminum or stainless steel) are available with integrated heating elements for precise temperature control (Model VC-LFR-18-H). They can be fabricated with other materials on special request.  The image to the right shows the chamber with an integrated heating element.

This chamber uses 1/16 ID tubing for the perfusion barbed ports and 1/16 inch ID tubing for the hose barb vacuum port.  The chamber is sold as a kit that included the chamber, o-rings, connectors and tubing. 

 

Laminar Flow Round, Models VC-LFR-18m, VC-LFR-18m-H

Model VC-LFR-18m

The model VC-LFR-18m and VC-LFR-18m-H chambers are similar to the VC-LFR-18 described above, but the chamber body is 0.75 inches in diameter as opposed to 1.21 inches. 

Although this Vacu-CellTM is designed for an 18 mm coverslip, it will adhere to a larger surface.  It will form a chamber with virtually any flat surface having a minimum of an 18 mm diameter.  The size of the flow chamber is 4.8 mm (length) x 8.0 mm (width) x 0.25 mm (depth).

The chambers made of metal (aluminum or stainless steel) are available with integrated heating elements for precise temperature control (Model VC-LFR-18-H). They can be fabricated with other materials on special request. 

This chamber uses 1/16 ID tubing for the perfusion barbed ports and 1/16 inch ID tubing for the hose barb vacuum port.  The chamber is sold as a kit that included the chamber, o-rings, connectors and tubing. 

 

 

 

Shear Stress Experiments

Several of our Vacu-CellTM chambers designs are ideally suited for experiments where application of a controlled shear stress cells on a coverslip surface is desired.  Details of how our chambers can be used for shear stress experiments are discussed in an Application Note.  Several of our customers have critically evaluated these chambers for use in shear stress experiments and have given us testimonials about their performance.  We invite you to read their comments. 

 

Chamber Specifications

The Laminar Flow Round chamber is designed to form a small rectangular flow chamber using a coverslip or other flat surface.  The specifications of the chamber are shown in the following table.

Chamber Dimension Specifications:

Model Number Length Width Thickness Laminar Flow Volume Total Volume*
VC-LFR-25 7.11 mm 9.58 mm 0.25 mm 17.4 µl 28.5 µl
VC-LFR-18 4.76 mm 7.94 mm 0.25 mm 9.7 µl 18.9 µl
VC-LFR-18m 4.76 mm 7.94 mm 0.25 mm 9.7 µl 18.9 µl

*The Total Volume refers to the Laminar Flow Volume (that portion of the chamber having a 0.25 mm thickness) plus the volume contained in the entrance and exit slots on either side of the laminar flow chamber.

This chamber is available in black anodized aluminum or stainless steel.  These chambers are ideal for epifluorescence and TIRF fluorescence experiments.  We also can make custom chamber configurations to your specifications.  Please contact us to discuss your application.  

See Custom Specs

Stage Holders

Stage holders are available for many popular inverted microscopes.  These holders gently clamp the Vacu-CellTM on the stage of the microscope using a single retaining spring clip.  The spring clip is easily moved to the side to insert the chamber into the holder using a thumb screw.  The stage holder is made of clear acrylic, allowing you to view the objectives below the microscope stage. 

 

Other Styles

We also manufacture other styles of Vacu-CellTM perfusion chambers for both circular and rectangular coverslips.  You can examine them on our product overview page.

Vacuum System

Vacuum Pump System (Model VCS-1)

We also offer a Vacuum Pump system for use with these perfusion chambers. The pump draws a vacuum of approximately -20 inches Hg and it fitted with a Luer-lock port for easy connectivity to standard tubing systems.  The Model VCS-1 vacuum pump assembly is approximately 8.4 (width) x 8.0 (depth) x 5.8 (height) inches in size.  These perfusion chambers also work well with other standard laboratory vacuum supplies.

Pricing Information

Temperature Controller

Temperature Controller (Model TC-30)

Model TC-30 Temperature ControllerWe provide a temperature controller designed to regulate the temperature of our Vacu-CellTM chambers.  The controller has several features designed for precise, noise-free temperature control.

 

Ordering Information, Chambers for 25mm round coverslip

Model Number Material
VC-LFR-25-SS Stainless Steel
VC-LFR-25-BA Black Anodized Aluminum
VC-LFR-25-SS-H Stainless Steel, with Heater
VC-LFR-25-BA-H Black Anodized Aluminum, with Heater
VC-25-ORINGS Replacement O-Rings (10 Sets)

Ordering Information, Chambers for 18mm round coverslip

Model Number Material
VC-LFR-18-SS Stainless Steel
VC-LFR-18-BA Black Anodized Aluminum
VC-LFR-18-SS-H Stainless Steel, with Heater
VC-LFR-18-BA-H Black Anodized Aluminum, with Heater
VC-LFR-18m-SS Stainless Steel
VC-LFR-18m-BA Black Anodized Aluminum
VC-LFR-18m-SS-H Stainless Steel, with Heater
VC-LFR-18m-BA-H Black Anodized Aluminum, with Heater
VC-18-ORINGS Replacement O-Rings (10 Sets)

Ordering Information, Stage Holders

Model Number Microscope
VC-RSH-OLY Olympus
VC-RSH-NIK Nikon
VC-RSH-ZEI Zeiss

(Specify microscope model when ordering)

Ordering Information, Coverslips

Model Number Description*
VC-25RCS-1 25 mm Round, No.1, Box of 160
VC-25RCS-2 25 mm Round, No.2, Box of 110
VC-18RCS-1 18 mm Round, No.1, Box of 310
VC-18RCS-2 18 mm Round, No.2, Box of 200

*No. 1, 0.13 - 0.16 mm thick
  No. 2, 0.19 - 0.23 mm thick

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