世联博研(北京)科技有限公司 主营:Flexcell细胞力学和regenhu细胞3D生物打印机销售技术服务: 美国Flexcell品牌FX-5000T细胞牵张应力加载培养系统,FX-5K细胞显微牵张应力加载培养系统,Tissue Train三维细胞组织培养与测试系统,FX-5000C三维细胞组织压应力加载培养系统,STR-4000细胞流体剪切应力加载培养系统,德国cellastix品牌Optical Stretcher高通量单细胞牵引应变与分析系统 Regenhu品牌3D discovery细胞友好型3D生物打印机,piuma细胞纳米压痕测试分析、aresis多点力学测试光镊,MagneTherm细胞肿瘤电磁热疗测试分析系统
服务电话: 010-67529703
主营产品: Flexcell细胞力学和regenhu细胞3D生物打印机销售技术服务: 美国Flexcell品牌FX-5000T细胞牵张应力加载培养系统,FX-5K细胞显微牵张应力加载培养系统,Tissue Train三维细胞组织培养与测试系统,FX-5000C三维细胞组织压应力加载培养系统,STR-4000细胞流体剪切应力加载培养系统,德国cellastix品牌Optical Stretcher高通量单细胞牵引应变与分析系统 Regenhu品牌3D discovery细胞友好型3D生物打印机,piuma细胞纳米压痕测试分析、aresis多点力学测试光镊,MagneTherm细胞肿瘤电磁热疗测试分析系统
联系我们
产品中心
所在位置:

ET RecAH0210S

  • 如果您对该产品感兴趣的话,可以
  • 产品名称:ET RecAH0210S
  • 产品型号:H0210S
  • 产品展商:其它品牌
  • 产品文档:无相关文档
简单介绍

RecA is a DNA binding protein with ssDNA-dependent ATPase activity. It plays vital roles in homologous recombination and DNA repair in bacteria by catalyzing strand exchange at a homologous sequence between single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) (1). ET RecAH0210SET RecAH0210S

产品描述

Description:

   


RecA is a DNA binding protein with ssDNA-dependent ATPase activity. It plays vital roles in homologous recombination and DNA repair in bacteria by catalyzing strand exchange at a homologous sequence between single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) (1). In the presence of ATP, the RecA protein first polymerizes on the single-stranded DNA to form a nucleoprotein filament. The nucleoprotein filament binds naked duplex DNA and randomly searches for homology. Once a homologous region is found, the strands are exchanged.

ET RecA (Extreme thermostable RecA) is an E. coli RecA homolog isolated from a hyperthermophilic microorganism. ET RecA has a ssDNA-dependent ATPase activity with an optimal temperature between 75 to 85ºC.

The extreme thermostability of ET RecA proves to be ideal for applications that require an elevated temperature condition, such as nucleic acid amplification and sequencing.

     
     

Source:

   


purified from an E. coli strain that overexpresses the recA gene isolated from a hyperthermophilic microorganism.
     
     

Applications:

   


Visualization of DNA structures for electron microscopy (2)
Site-directed mutagenesis through D-loop (3,4)
Screening of DNA libraries using RecA-probe filaments (5,6)
Targeted cleavage of DNA (7)
Improvement of PCR specificity and yield (8)

     
     
     

Storage Conditions:

   


Concentration:

1mg/ml

Storage Buffer:
10 mM Tris-HCl
100 mM KCl
0.1 mM EDTA
1 mM DTT
0.1% Triton X-100
50% Glycerol
pH7.5 @ 25ºC

Storage Temperature:
-20ºC

     
   

Quality Control:

 


Quality Assurance Statement:

ET RecA is purified free of contaminating endonucleases and exonucleases. Each lot is tested for single-stranded DNA-dependent ATPase activity and is visually determined to be > 95% pure on an SDS-polyacrylamide gel.

Exonuclease Activity:
Incubation of 20 µg ET RecA for 4 hours at 37°C in 50 µl reaction buffer containing 50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate and 1 mM dithiothreitol, pH 7.9 @ 25°C, with 1 µg of a mixture of single and double-stranded [3H] E. coli DNA (200,000 cpm/µg) released < 0.05% of the total radioactivity.

Endonuclease Assay:
Incubation of 10 µg ET RecA for 4 hours at 37°C in 50 µl reaction buffer containing 50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate and 1 mM dithiothreitol, pH 7.9 @ 25°C, with 1 µg ΦX174 RF I DNA gave < 5% conversion to RF II.

Nuclease Activity:
Incubation of 20 µg ET RecA for 16 hours at 37°C in 50 µl of reaction buffer containing 50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate and 1 mM dithiothreitol, pH 7.9 @ 25°C, with 1 µg λ DNA yielded a clear and sharp band on an agarose gel.

     
   

References:

 


  1. Radding, C. M. (1991) J Biol Chem 266, 5355-8.
  2. Wasserman, S. A. & Cozzarelli, N. R. (1985) Proc Natl Acad Sci U S A 82, 1079-83.
  3. Biet, E., Maurisse, R., Dutreix, M. & Sun, J. (2001) Biochemistry 40, 1779-86.
  4. Shortle, D., Koshland, D., Weinstock, G. M. & Botstein, D. (1980) Proc Natl Acad Sci U S A 77, 5375-9.
  5. Rigas, B., Welcher, A. A., Ward, D. C. & Weissman, S. M. (1986) Proc Natl Acad Sci U S A 83, 9591-5.
  6. Honigberg, S. M., Rao, B. J. & Radding, C. M. (1986) Proc Natl Acad Sci U S A 83, 9586-90.
  7. Koob, M., Burkiewicz, A., Kur, J. & Szybalski, W. (1992) Nucleic Acids Res 20, 5831-6.
  8. Shigemori, Y., Mikawa, T., Shibata, T. & Oishi, M. (2005) Nucleic Acids Res 33, e126.
  9. ET RecAH0210SET RecAH0210S
ET RecAH0210SET RecAH0210SET RecAH0210S
产品留言
标题
联系人
联系电话
内容
验证码
点击换一张
注:1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!
Copyright@ 2003-2024  世联博研(北京)科技有限公司版权所有      电话:13466675923 传真: 地址:北京市海淀区西三旗上奥世纪中心A座9层906 邮编:100096