ET SSB PlusH0230S 世联博研（北京）科技有限公司

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产品名称：ET SSB PlusH0230S
产品型号：H0230S
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简单介绍

ET SSB Plus, (formally known as Primer Navigator™) is a mixture of thermostable RecA and thermostable SSB that is optimized for maximal enhancement of nucleic acid amplification, including HDA and PCR. SSB and RecA have been shown to improve the specificity and yield of RT-PCR, PCR and DNA sequencing (1-7).ET SSB PlusH0230SET SSB PlusH0230S

产品描述

Description:

*ET SSB Plus, (formally known as Primer Navigator™)* is a mixture of thermostable RecA and thermostable SSB that is optimized for maximal enhancement of nucleic acid amplification, including HDA and PCR. SSB and RecA have been shown to improve the specificity and yield of RT-PCR, PCR and DNA sequencing (1-7). SSB proteins destabilize DNA secondary structure and increase the processivity of DNA polymerases (8) while RecA proteins bind to ssDNA and search for homology in dsDNA (9). SSBs have also been shown to enhance RecA function in ssDNA binding and strand transfer (10,11). ET SSB Plus has been developed to maximize their effect on difficult amplification reactions such as amplification of G+C rich region or multiplex PCR.


Reagent supplied:

Solution A (50X)


Reaction condition:

Add 1 or 2U ET SSB Plus and 1 µl of Solution A in a 50 µl reaction.




Storage Conditions:

Concentration: 2U/µl Storage Buffer: 10 mM Tris-HCl 100 mM KCl 0.1 mM EDTA 1 mM DTT 0.1% Triton X-100 50% Glycerol pH7.5 @ 25ºC Storage Temperature: -20ºC


References:

Shigemori, Y., Mikawa, T., Shibata, T. & Oishi, M. (2005) Nucleic Acids Res 33, e126. Villalva, C., Touriol, C., Seurat, P., Trempat, P., Delsol, G. & Brousset, P. (2001) Biotechniques 31, 81-3, 86. Schwarz, K., Hansen-Hagge, T. & Bartram, C. (1990) Nucleic Acids Res 18, 1079. Chou, Q. (1992) Nucleic Acids Res 20, 4371. Oshima, R. G. (1992) Biotechniques 13, 188. Rapley, R. (1994) Mol Biotechnol 2, 295-8. Olszewski, M., Rebala, K., Szczerkowska, Z. & Kur, J. (2005) Mol Cell Probes 19, 203-5. Myers, T. W. & Romano, L. J. (1988) J Biol Chem 263, 17006-15. Radding, C. M. (1991) J Biol Chem 266, 5355-8. Reddy, M. S., Vaze, M. B., Madhusudan, K. & Muniyappa, K. (2000) Biochemistry 39, 14250-62. West, S. C., Cassuto, E. & Howard-Flanders, P. (1982) Mol Gen Genet 186, 333-8. ET SSB PlusH0230SET SSB PlusH0230SET SSB PlusH0230SET SSB PlusH0230SET SSB PlusH0230S

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