Description:
The T7 Gp4A Helicase exhibits a 5’ to 3’ helicase activity as well as primase activity. It is highly processive, traveling an average of 75 kb of single stranded DNA before dissociating.
SUPPLIED WITH: 10X Gp4A Reaction Buffer Supplied by Users: dTTP
Source:
Reaction condition:
1X Gp4A Reaction Buffer: 35 mM Tris–acetate, pH 7.5 11 mM magnesium acetate 5 mM DTT 0.01% Triton X-100 Supplement with 2 mM dTTP. Incubate at 25 - 37ºC. NOTE: The concentration of dTTP should be optimized for each specific application. For the primase activity, supplement reactions with NTPs.
Storage Conditions:
Quality Control:
Quality Assurance Statement: T7 Gp4A Helicase is purified free of contaminating endonucleases and exonucleases. Each lot is tested for DNA unwinding activity in functional activity assays.T7 Gp4A HelicaseH0250S,