biohelixH0300SbiohelixH0300SWhole Genome Amplification (WGA) techniques are a useful tool for cancer and genetic research, and typically used to amplify genomic DNA for archiving and down-stream analysis including genotyping, forensics, comparative genomic hybridization, and single cell analysis.
The Rapisome™ pWGA kit non-specifically amplifies total DNA. Unlike other WGA products, the Rapisome™ pWGA kit is based upon the in vitro reconstitution of naturally existing cellular DNA replication system, which performs fast isothermal DNA amplification without the need for thermocycling, prior heat-denaturation, or added primers. The pWGA technology uses a primase to synthesize primers on-site, generating multiple initiation sites for random, whole genome amplification. Therefore, there is no need to add random primers to the reaction.
The pWGA technology also utilizes a helicase to denature double-stranded template DNA, eliminating the initial heating step that is required by other WGA technologies. Thus, the Rapisome™ pWGA reaction is truly isothermal.
pWGA is also faster than any other commercially available WGA techniques : the reaction time for pWGA is less than one hour. This makes Rapisome™ pWGA attractive technology for use in diagnostic applications more so than other conventional methods.biohelixH0300S
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