世联博研（北京）科技有限公司 主营：Flexcell细胞力学和regenhu细胞3D生物打印机销售技术服务： 美国Flexcell品牌FX-5000T细胞牵张应力加载培养系统，FX-5K细胞显微牵张应力加载培养系统，Tissue Train三维细胞组织培养与测试系统，FX-5000C三维细胞组织压应力加载培养系统，STR-4000细胞流体剪切应力加载培养系统，德国cellastix品牌Optical Stretcher高通量单细胞牵引应变与分析系统 Regenhu品牌3D discovery细胞友好型3D生物打印机,piuma细胞纳米压痕测试分析、aresis多点力学测试光镊，MagneTherm细胞肿瘤电磁热疗测试分析系统
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主营产品： Flexcell细胞力学和regenhu细胞3D生物打印机销售技术服务： 美国Flexcell品牌FX-5000T细胞牵张应力加载培养系统，FX-5K细胞显微牵张应力加载培养系统，Tissue Train三维细胞组织培养与测试系统，FX-5000C三维细胞组织压应力加载培养系统，STR-4000细胞流体剪切应力加载培养系统，德国cellastix品牌Optical Stretcher高通量单细胞牵引应变与分析系统 Regenhu品牌3D discovery细胞友好型3D生物打印机,piuma细胞纳米压痕测试分析、aresis多点力学测试光镊，MagneTherm细胞肿瘤电磁热疗测试分析系统
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活体单细胞毒性荧光检测试剂盒,Live, Single Cell-based Fluorogenic Cytotoxicity Assay Assay Kit

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  • 产品名称：活体单细胞毒性荧光检测试剂盒,Live, Single Cell-based Fluorogenic Cytotoxicity Assay Assay Kit
  • 产品型号：CyToxiLux® , GranToxiLux®, and PanToxiLux™
  • 产品展商：phiphilux
  • 产品文档：无相关文档
简单介绍

CyToxiLux® , GranToxiLux®, and PanToxiLux™ Live, Single Cell-based Fluorogenic Cytotoxicity Assay Assay Kits A tumor cell is engaged and then "killed" by a cytotoxic lymphocyte Principle of assays: Following the successful delivery of a lethal hit by cytotoxic lymphocytes, protease activities leading to cell death in individual target cells can be measured by flow cytometry or fluorescence mi

产品描述


CyToxiLux® , GranToxiLux®, and PanToxiLux™


Live, Single Cell-based Fluorogenic Cytotoxicity Assay Assay Kits

A tumor cell is engaged and then "killed" by a cytotoxic lymphocyte

Principle of assays:
Following the successful delivery of a lethal hit by cytotoxic lymphocytes, protease activities leading to cell death in individual target cells can be measured by flow cytometry or fluorescence microscopy. In each kit cleavage of a cell permeable fluorogenic substrate reports the following activities:

CyToxiLux® : downstream caspase
GranToxiLux®: granzyme B
PanToxiLux™: granzyme B and upstream caspase

 

Measurements with CyToxiLux®, GranToxiLux®, and PanToxiLuxTM are superior to bulk assays e.g., LDH and 51Cr release, in terms of both time (0.3-2.0 hr. vs. 4 hr.) and sensitivity (relatively weak CTL responses against subdominant epitopes are detectable).

Detailed CyToxiLux® PLUS! instructions are available for download in PDF format
Detailed GranToxiLux® PLUS! instructions are available for download in PDF format
Detailed PanToxiLux® instructions are available for download in PDF format
Detailed PanToxiLux™ P2D2 instructions are available for download in PDF format


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