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主营产品: Flexcell细胞力学和regenhu细胞3D生物打印机销售技术服务: 美国Flexcell品牌FX-5000T细胞牵张应力加载培养系统,FX-5K细胞显微牵张应力加载培养系统,Tissue Train三维细胞组织培养与测试系统,FX-5000C三维细胞组织压应力加载培养系统,STR-4000细胞流体剪切应力加载培养系统,德国cellastix品牌Optical Stretcher高通量单细胞牵引应变与分析系统 Regenhu品牌3D discovery细胞友好型3D生物打印机,piuma细胞纳米压痕测试分析、aresis多点力学测试光镊,MagneTherm细胞肿瘤电磁热疗测试分析系统
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Nitric Oxide Measurement System ,一氧化氮测量系统

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  • 产品名称:Nitric Oxide Measurement System ,一氧化氮测量系统
  • 产品型号:ArrowSTRAIGHT
  • 产品展商:Lazar世联博研公司
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简单介绍

Nitric Oxide Measurement System ,一氧化氮测量系统Nitric Oxide Measurement System ,一氧化氮测量系统Measure NO in Your Smallest Samples Plasma Serum Urine Whole blood Saliva Tissue homogenates Cell Cultures

产品描述

Nitric Oxide Measurement System ,一氧化氮测量系统Nitric Oxide Measurement System ,一氧化氮测量系统Nitric oxide (NO) plays a major role in a variety of mammalian biological processes including blood pressure homeostasis, immune regulation, and nervous system signal transmission. NO is very unstable and has a physiological half life of only 1 to 40 seconds. Even though there are electrochemical (amperometric) methods for detecting NO, its rapid degradation to other nitrogen oxide compounds makes it difficult to determine quantitatively with a high degree of accuracy. The amperometric method for assay of NO is expensive, time consuming, and operator dependent. NO oxidizes into two stable end products, namely nitrite (NO2-) and nitrate (NO3-) ions. The concentration of these two end products can be used to quantify NO production without the measurement problems caused by the transient nature of NO.

There are various methods for quantitatively determining the concentration of both NO2- and NO3- ions. The most common methods involved the use of the Griess reagent which reacts with NO2- ion to produce a stable azo end product which is purple in color and can be quantified using colorimeteric or spectrophotometric analytical techniques. There are three basic problems with this colorimetric technique. First of all, the Griess reagent only reacts with the NO2- compound thereby requiring an additional difficult step to completely reduce NO3- ion to NO2-. Secondly, like with all colorimetric methods, the Griess technique can be affected by original sample color or turbidity. Thirdly, this method is very time consuming and tedious because it involved several intermediate chemical reactions.

Recently a potentially more accurate, less time consuming, and less tedious method has been developed for assaying both NO2- and NO3- which relies on the use of ion selective electrodes for both NO2- and NO3- ions. These electrodes have been miniaturized to be able to measure samples down to 50 to 100 microliters with detection limits down to the low microMolar range for both species. Since both NO2- and NO3- ions can be measured individually no reaction to reduce NO3- to NO2- is required. This micro electrode system comes complete with a sophisticated ion analyzer which attaches via RS232 or USB port to a PC or laptop to give accurate concentration measurements for both ions.Nitric Oxide Measurement System ,一氧化氮测量系统Nitric Oxide Measurement System ,一氧化氮测量系统Nitric Oxide Measurement System ,一氧化氮测量系统

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